File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of an attenuated SARS-CoV-2 variant with a deletion at the S1/S2 junction of the spike protein

TitleCharacterization of an attenuated SARS-CoV-2 variant with a deletion at the S1/S2 junction of the spike protein
Authors
Issue Date2021
PublisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html
Citation
Nature Communications, 2021, v. 12 n. 1, p. article no. 2790 How to Cite?
AbstractSARS-CoV-2 is of zoonotic origin and contains a PRRA polybasic cleavage motif which is considered critical for efficient infection and transmission in humans. We previously reported on a panel of attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction of the spike protein. Here, we characterize pathogenicity, immunogenicity, and protective ability of a further cell-adapted SARS-CoV-2 variant, Ca-DelMut, in in vitro and in vivo systems. Ca-DelMut replicates more efficiently than wild type or parental virus in Vero E6 cells, but causes no apparent disease in hamsters, despite replicating in respiratory tissues. Unlike wild type virus, Ca-DelMut causes no obvious pathological changes and does not induce elevation of proinflammatory cytokines, but still triggers a strong neutralizing antibody and T cell response in hamsters and mice. Ca-DelMut immunized hamsters challenged with wild type SARS-CoV-2 are fully protected, with little sign of virus replication in the upper or lower respiratory tract, demonstrating sterilizing immunity.
Persistent Identifierhttp://hdl.handle.net/10722/305002
ISSN
2023 Impact Factor: 14.7
2023 SCImago Journal Rankings: 4.887
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, P-
dc.contributor.authorLau, SY-
dc.contributor.authorDeng, S-
dc.contributor.authorChen, P-
dc.contributor.authorMok, BWY-
dc.contributor.authorZhang, AJ-
dc.contributor.authorLee, ACY-
dc.contributor.authorChan, KH-
dc.contributor.authorTam, RCY-
dc.contributor.authorXU, H-
dc.contributor.authorZhou, R-
dc.contributor.authorSong, W-
dc.contributor.authorLiu, L-
dc.contributor.authorTo, KKW-
dc.contributor.authorChan, JFW-
dc.contributor.authorChen, Z-
dc.contributor.authorYuen, KY-
dc.contributor.authorChen, H-
dc.date.accessioned2021-10-05T02:38:20Z-
dc.date.available2021-10-05T02:38:20Z-
dc.date.issued2021-
dc.identifier.citationNature Communications, 2021, v. 12 n. 1, p. article no. 2790-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10722/305002-
dc.description.abstractSARS-CoV-2 is of zoonotic origin and contains a PRRA polybasic cleavage motif which is considered critical for efficient infection and transmission in humans. We previously reported on a panel of attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction of the spike protein. Here, we characterize pathogenicity, immunogenicity, and protective ability of a further cell-adapted SARS-CoV-2 variant, Ca-DelMut, in in vitro and in vivo systems. Ca-DelMut replicates more efficiently than wild type or parental virus in Vero E6 cells, but causes no apparent disease in hamsters, despite replicating in respiratory tissues. Unlike wild type virus, Ca-DelMut causes no obvious pathological changes and does not induce elevation of proinflammatory cytokines, but still triggers a strong neutralizing antibody and T cell response in hamsters and mice. Ca-DelMut immunized hamsters challenged with wild type SARS-CoV-2 are fully protected, with little sign of virus replication in the upper or lower respiratory tract, demonstrating sterilizing immunity.-
dc.languageeng-
dc.publisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html-
dc.relation.ispartofNature Communications-
dc.rightsNature Communications. Copyright © Nature Research: Fully open access journals.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleCharacterization of an attenuated SARS-CoV-2 variant with a deletion at the S1/S2 junction of the spike protein-
dc.typeArticle-
dc.identifier.emailWang, P: puiwang@hkucc.hku.hk-
dc.identifier.emailMok, BWY: bobomok@hku.hk-
dc.identifier.emailZhang, AJ: zhangajx@hkucc.hku.hk-
dc.identifier.emailLee, ACY: cyalee@hku.hk-
dc.identifier.emailChan, KH: chankh2@hkucc.hku.hk-
dc.identifier.emailTam, RCY: rach2011@hku.hk-
dc.identifier.emailZhou, R: zhourh@hku.hk-
dc.identifier.emailSong, W: wjsong@hkucc.hku.hk-
dc.identifier.emailLiu, L: liuli71@hkucc.hku.hk-
dc.identifier.emailTo, KKW: kelvinto@hku.hk-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailChen, Z: zchenai@hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.emailChen, H: hlchen@hku.hk-
dc.identifier.authorityZhang, AJ=rp00413-
dc.identifier.authorityChan, KH=rp01921-
dc.identifier.authorityLiu, L=rp00268-
dc.identifier.authorityTo, KKW=rp01384-
dc.identifier.authorityChan, JFW=rp01736-
dc.identifier.authorityChen, Z=rp00243-
dc.identifier.authorityYuen, KY=rp00366-
dc.identifier.authorityChen, H=rp00383-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41467-021-23166-0-
dc.identifier.pmid33986286-
dc.identifier.pmcidPMC8119425-
dc.identifier.scopuseid_2-s2.0-85105806346-
dc.identifier.hkuros326079-
dc.identifier.volume12-
dc.identifier.issue1-
dc.identifier.spagearticle no. 2790-
dc.identifier.epagearticle no. 2790-
dc.identifier.isiWOS:000657822800025-
dc.publisher.placeUnited Kingdom-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats