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Article: Efficacy of the dual-action GA-KR12 peptide for remineralising initial enamel caries: an in vitro study

TitleEfficacy of the dual-action GA-KR12 peptide for remineralising initial enamel caries: an in vitro study
Authors
Issue Date2021
Citation
Clinical Oral Investigations, 2021 How to Cite?
AbstractObjective: To investigate the antibiofilm and remineralising effects of the dual-action peptide GA-KR12 on artificial enamel caries. Materials and methods: Enamel blocks with artificial caries were treated with sterilised deionised water as control or GA-KR12. The blocks underwent biochemical cycling with Streptococcus mutans for 3 weeks. The architecture, viability, and growth kinetics of the biofilm were determined, respectively, by scanning electron microscopy (SEM), confocal laser scanning microscopy, and quantitative (culture colony-forming units, CFUs). The mineral loss, calcium-to-phosphorus ratio, surface morphology, and crystal characteristics of the enamel surface were determined, respectively, using micro-computed tomography, energy dispersive spectroscopy, SEM, and X-ray diffraction (XRD). Results: SEM showed confluent growth of S. mutans in the control group but not in the GA-KR12-treated group. The deadto-live ratios of the control and GA-KR12-treated groups were 0.42 ± 0.05 and 0.81 ± 0.08, respectively (p < 0.001). The log CFUs of the control and GA-KR12-treated groups were 8.15 ± 0.32 and 6.70 ± 0.49, respectively (p < 0.001). The mineral losses of the control and GA-KR12-treated groups were 1.39 ± 0.09 gcm−3 and 1.19 ± 0.05 gcm−3, respectively (p < 0.001). The calcium-to-phosphorus molar ratios of the control and GA-KR12-treated groups were 1.47 ± 0.03 and 1.57 ± 0.02, respectively (p < 0.001). A uniformly remineralised prismatic pattern on enamel blocks was observed in the GA-KR12-treated but not in the control group. The hydroxyapatite in the GA-KR12-treated group was better crystallised than that in the control group. Conclusion: The dual-action peptide GA-KR12 inhibited the growth of S. mutans biofilm and promoted the remineralization of enamel caries. Clinical relevance: GA-KR12 potentially is applicable for managing enamel caries.
Persistent Identifierhttp://hdl.handle.net/10722/308059
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNiu, JY-
dc.contributor.authorYin, X-
dc.contributor.authorWu, WKK-
dc.contributor.authorLi, QL-
dc.contributor.authorMei, L-
dc.contributor.authorChu, CH-
dc.date.accessioned2021-11-12T13:41:55Z-
dc.date.available2021-11-12T13:41:55Z-
dc.date.issued2021-
dc.identifier.citationClinical Oral Investigations, 2021-
dc.identifier.urihttp://hdl.handle.net/10722/308059-
dc.description.abstractObjective: To investigate the antibiofilm and remineralising effects of the dual-action peptide GA-KR12 on artificial enamel caries. Materials and methods: Enamel blocks with artificial caries were treated with sterilised deionised water as control or GA-KR12. The blocks underwent biochemical cycling with Streptococcus mutans for 3 weeks. The architecture, viability, and growth kinetics of the biofilm were determined, respectively, by scanning electron microscopy (SEM), confocal laser scanning microscopy, and quantitative (culture colony-forming units, CFUs). The mineral loss, calcium-to-phosphorus ratio, surface morphology, and crystal characteristics of the enamel surface were determined, respectively, using micro-computed tomography, energy dispersive spectroscopy, SEM, and X-ray diffraction (XRD). Results: SEM showed confluent growth of S. mutans in the control group but not in the GA-KR12-treated group. The deadto-live ratios of the control and GA-KR12-treated groups were 0.42 ± 0.05 and 0.81 ± 0.08, respectively (p < 0.001). The log CFUs of the control and GA-KR12-treated groups were 8.15 ± 0.32 and 6.70 ± 0.49, respectively (p < 0.001). The mineral losses of the control and GA-KR12-treated groups were 1.39 ± 0.09 gcm−3 and 1.19 ± 0.05 gcm−3, respectively (p < 0.001). The calcium-to-phosphorus molar ratios of the control and GA-KR12-treated groups were 1.47 ± 0.03 and 1.57 ± 0.02, respectively (p < 0.001). A uniformly remineralised prismatic pattern on enamel blocks was observed in the GA-KR12-treated but not in the control group. The hydroxyapatite in the GA-KR12-treated group was better crystallised than that in the control group. Conclusion: The dual-action peptide GA-KR12 inhibited the growth of S. mutans biofilm and promoted the remineralization of enamel caries. Clinical relevance: GA-KR12 potentially is applicable for managing enamel caries.-
dc.languageeng-
dc.relation.ispartofClinical Oral Investigations-
dc.titleEfficacy of the dual-action GA-KR12 peptide for remineralising initial enamel caries: an in vitro study-
dc.typeArticle-
dc.identifier.emailYin, X: irisxyin@hku.hk-
dc.identifier.emailMei, L: mei1123@hku.hk-
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.authorityMei, L=rp01840-
dc.identifier.authorityChu, CH=rp00022-
dc.identifier.doi10.1007/s00784-021-04210-1-
dc.identifier.scopuseid_2-s2.0-85116886498-
dc.identifier.hkuros329700-
dc.identifier.isiWOS:000706023400001-

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