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- Publisher Website: 10.1007/s00784-021-04210-1
- Scopus: eid_2-s2.0-85116886498
- PMID: 34635946
- WOS: WOS:000706023400001
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Article: Efficacy of the dual-action GA-KR12 peptide for remineralising initial enamel caries: an in vitro study
Title | Efficacy of the dual-action GA-KR12 peptide for remineralising initial enamel caries: an in vitro study |
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Authors | |
Keywords | Antimicrobial Caries Demineralisation Enamel Peptides Prevention Remineralisation |
Issue Date | 2022 |
Citation | Clinical Oral Investigations, 2022, v. 26 n. 3, p. 2441-2451 How to Cite? |
Abstract | Objective: To investigate the antibiofilm and remineralising effects of the dual-action peptide GA-KR12 on artificial enamel caries. Materials and methods: Enamel blocks with artificial caries were treated with sterilised deionised water as control or GA-KR12. The blocks underwent biochemical cycling with Streptococcus mutans for 3 weeks. The architecture, viability, and growth kinetics of the biofilm were determined, respectively, by scanning electron microscopy (SEM), confocal laser scanning microscopy, and quantitative (culture colony-forming units, CFUs). The mineral loss, calcium-to-phosphorus ratio, surface morphology, and crystal characteristics of the enamel surface were determined, respectively, using micro-computed tomography, energy dispersive spectroscopy, SEM, and X-ray diffraction (XRD). Results: SEM showed confluent growth of S. mutans in the control group but not in the GA-KR12-treated group. The deadto-live ratios of the control and GA-KR12-treated groups were 0.42 ± 0.05 and 0.81 ± 0.08, respectively (p < 0.001). The log CFUs of the control and GA-KR12-treated groups were 8.15 ± 0.32 and 6.70 ± 0.49, respectively (p < 0.001). The mineral losses of the control and GA-KR12-treated groups were 1.39 ± 0.09 gcm−3 and 1.19 ± 0.05 gcm−3, respectively (p < 0.001). The calcium-to-phosphorus molar ratios of the control and GA-KR12-treated groups were 1.47 ± 0.03 and 1.57 ± 0.02, respectively (p < 0.001). A uniformly remineralised prismatic pattern on enamel blocks was observed in the GA-KR12-treated but not in the control group. The hydroxyapatite in the GA-KR12-treated group was better crystallised than that in the control group. Conclusion: The dual-action peptide GA-KR12 inhibited the growth of S. mutans biofilm and promoted the remineralization of enamel caries. Clinical relevance: GA-KR12 potentially is applicable for managing enamel caries. |
Persistent Identifier | http://hdl.handle.net/10722/308059 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.942 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Niu, JY | - |
dc.contributor.author | Yin, X | - |
dc.contributor.author | Wu, WKK | - |
dc.contributor.author | Li, QL | - |
dc.contributor.author | Mei, L | - |
dc.contributor.author | Chu, CH | - |
dc.date.accessioned | 2021-11-12T13:41:55Z | - |
dc.date.available | 2021-11-12T13:41:55Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Clinical Oral Investigations, 2022, v. 26 n. 3, p. 2441-2451 | - |
dc.identifier.issn | 1432-6981 | - |
dc.identifier.uri | http://hdl.handle.net/10722/308059 | - |
dc.description.abstract | Objective: To investigate the antibiofilm and remineralising effects of the dual-action peptide GA-KR12 on artificial enamel caries. Materials and methods: Enamel blocks with artificial caries were treated with sterilised deionised water as control or GA-KR12. The blocks underwent biochemical cycling with Streptococcus mutans for 3 weeks. The architecture, viability, and growth kinetics of the biofilm were determined, respectively, by scanning electron microscopy (SEM), confocal laser scanning microscopy, and quantitative (culture colony-forming units, CFUs). The mineral loss, calcium-to-phosphorus ratio, surface morphology, and crystal characteristics of the enamel surface were determined, respectively, using micro-computed tomography, energy dispersive spectroscopy, SEM, and X-ray diffraction (XRD). Results: SEM showed confluent growth of S. mutans in the control group but not in the GA-KR12-treated group. The deadto-live ratios of the control and GA-KR12-treated groups were 0.42 ± 0.05 and 0.81 ± 0.08, respectively (p < 0.001). The log CFUs of the control and GA-KR12-treated groups were 8.15 ± 0.32 and 6.70 ± 0.49, respectively (p < 0.001). The mineral losses of the control and GA-KR12-treated groups were 1.39 ± 0.09 gcm−3 and 1.19 ± 0.05 gcm−3, respectively (p < 0.001). The calcium-to-phosphorus molar ratios of the control and GA-KR12-treated groups were 1.47 ± 0.03 and 1.57 ± 0.02, respectively (p < 0.001). A uniformly remineralised prismatic pattern on enamel blocks was observed in the GA-KR12-treated but not in the control group. The hydroxyapatite in the GA-KR12-treated group was better crystallised than that in the control group. Conclusion: The dual-action peptide GA-KR12 inhibited the growth of S. mutans biofilm and promoted the remineralization of enamel caries. Clinical relevance: GA-KR12 potentially is applicable for managing enamel caries. | - |
dc.language | eng | - |
dc.relation.ispartof | Clinical Oral Investigations | - |
dc.subject | Antimicrobial | - |
dc.subject | Caries | - |
dc.subject | Demineralisation | - |
dc.subject | Enamel | - |
dc.subject | Peptides | - |
dc.subject | Prevention | - |
dc.subject | Remineralisation | - |
dc.title | Efficacy of the dual-action GA-KR12 peptide for remineralising initial enamel caries: an in vitro study | - |
dc.type | Article | - |
dc.identifier.email | Yin, X: irisxyin@hku.hk | - |
dc.identifier.email | Mei, L: mei1123@hku.hk | - |
dc.identifier.email | Chu, CH: chchu@hku.hk | - |
dc.identifier.authority | Mei, L=rp01840 | - |
dc.identifier.authority | Chu, CH=rp00022 | - |
dc.identifier.doi | 10.1007/s00784-021-04210-1 | - |
dc.identifier.pmid | 34635946 | - |
dc.identifier.scopus | eid_2-s2.0-85116886498 | - |
dc.identifier.hkuros | 329700 | - |
dc.identifier.volume | 26 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 2441 | - |
dc.identifier.epage | 2451 | - |
dc.identifier.isi | WOS:000706023400001 | - |