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Article: Characterization of the humoral immune response to the EBV proteome in extranodal NK/T-cell lymphoma

TitleCharacterization of the humoral immune response to the EBV proteome in extranodal NK/T-cell lymphoma
Authors
Issue Date2021
PublisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2021, v. 11 n. 1, p. article no. 23664 How to Cite?
AbstractExtranodal natural killer/T-cell lymphoma (NKTCL) is an aggressive malignancy that has been etiologically linked to Epstein-Barr virus (EBV) infection, with EBV gene transcripts identified in almost all cases. However, the humoral immune response to EBV in NKTCL patients has not been well characterized. We examined the antibody response to EBV in plasma samples from 51 NKTCL cases and 154 controls from Hong Kong and Taiwan who were part of the multi-center, hospital-based AsiaLymph case–control study. The EBV-directed serological response was characterized using a protein microarray that measured IgG and IgA antibodies against 202 protein sequences representing the entire EBV proteome. We analyzed 157 IgG antibodies and 127 IgA antibodies that fulfilled quality control requirements. Associations between EBV serology and NKTCL status were disproportionately observed for IgG rather than IgA antibodies. Nine anti-EBV IgG responses were significantly elevated in NKTCL cases compared with controls and had ORshighest vs. lowest tertile > 6.0 (Bonferroni-corrected P-values < 0.05). Among these nine elevated IgG responses in NKTCL patients, three IgG antibodies (all targeting EBNA3A) are novel and have not been observed for other EBV-associated tumors of B-cell or epithelial origin. IgG antibodies against EBNA1, which have consistently been elevated in other EBV-associated tumors, were not elevated in NKTCL cases. We characterize the antibody response against EBV for patients with NKTCL and identify IgG antibody responses against six distinct EBV proteins. Our findings suggest distinct serologic patterns of this NK/T-cell lymphoma compared with other EBV-associated tumors of B-cell or epithelial origin.
Persistent Identifierhttp://hdl.handle.net/10722/309850
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 0.900
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, Z-
dc.contributor.authorSarathkumara, YD-
dc.contributor.authorChan, JKC-
dc.contributor.authorKwong, YL-
dc.contributor.authorLam, TH-
dc.contributor.authorIp, DKM-
dc.contributor.authorChiu, BCH-
dc.contributor.authorXu, J-
dc.contributor.authorSu, YC-
dc.contributor.authorProietti, C-
dc.contributor.authorCooper, MM-
dc.contributor.authorYu, KJ-
dc.contributor.authorBassig, B-
dc.contributor.authorLiang, R-
dc.contributor.authorHu, W-
dc.contributor.authorJi, BT-
dc.contributor.authorCoghill, AE-
dc.contributor.authorPfeiffer, RM-
dc.contributor.authorHildesheim, A-
dc.contributor.authorRothman, N-
dc.contributor.authorDoolan, DL-
dc.contributor.authorLan, Q-
dc.date.accessioned2022-01-10T09:14:42Z-
dc.date.available2022-01-10T09:14:42Z-
dc.date.issued2021-
dc.identifier.citationScientific Reports, 2021, v. 11 n. 1, p. article no. 23664-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/309850-
dc.description.abstractExtranodal natural killer/T-cell lymphoma (NKTCL) is an aggressive malignancy that has been etiologically linked to Epstein-Barr virus (EBV) infection, with EBV gene transcripts identified in almost all cases. However, the humoral immune response to EBV in NKTCL patients has not been well characterized. We examined the antibody response to EBV in plasma samples from 51 NKTCL cases and 154 controls from Hong Kong and Taiwan who were part of the multi-center, hospital-based AsiaLymph case–control study. The EBV-directed serological response was characterized using a protein microarray that measured IgG and IgA antibodies against 202 protein sequences representing the entire EBV proteome. We analyzed 157 IgG antibodies and 127 IgA antibodies that fulfilled quality control requirements. Associations between EBV serology and NKTCL status were disproportionately observed for IgG rather than IgA antibodies. Nine anti-EBV IgG responses were significantly elevated in NKTCL cases compared with controls and had ORshighest vs. lowest tertile > 6.0 (Bonferroni-corrected P-values < 0.05). Among these nine elevated IgG responses in NKTCL patients, three IgG antibodies (all targeting EBNA3A) are novel and have not been observed for other EBV-associated tumors of B-cell or epithelial origin. IgG antibodies against EBNA1, which have consistently been elevated in other EBV-associated tumors, were not elevated in NKTCL cases. We characterize the antibody response against EBV for patients with NKTCL and identify IgG antibody responses against six distinct EBV proteins. Our findings suggest distinct serologic patterns of this NK/T-cell lymphoma compared with other EBV-associated tumors of B-cell or epithelial origin.-
dc.languageeng-
dc.publisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleCharacterization of the humoral immune response to the EBV proteome in extranodal NK/T-cell lymphoma-
dc.typeArticle-
dc.identifier.emailKwong, YL: ylkwong@hkucc.hku.hk-
dc.identifier.emailLam, TH: hrmrlth@HKUCC-COM.hku.hk-
dc.identifier.emailIp, DKM: dkmip@hku.hk-
dc.identifier.emailXu, J: xusunjun@hku.hk-
dc.identifier.emailLiang, R: rliang@hkucc.hku.hk-
dc.identifier.authorityKwong, YL=rp00358-
dc.identifier.authorityLam, TH=rp00326-
dc.identifier.authorityIp, DKM=rp00256-
dc.identifier.authorityLiang, R=rp00345-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41598-021-02788-w-
dc.identifier.pmid34880297-
dc.identifier.pmcidPMC8655014-
dc.identifier.scopuseid_2-s2.0-85120966945-
dc.identifier.hkuros331395-
dc.identifier.volume11-
dc.identifier.issue1-
dc.identifier.spagearticle no. 23664-
dc.identifier.epagearticle no. 23664-
dc.identifier.isiWOS:000734524200049-
dc.publisher.placeUnited Kingdom-

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