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Article: Light-sheet microscopy in the near-infrared II window

TitleLight-sheet microscopy in the near-infrared II window
Authors
Issue Date2019
Citation
Nature Methods, 2019, v. 16, n. 6, p. 545-552 How to Cite?
AbstractNon-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000–1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,700 nm, respectively, for optical sectioning at a penetration depth of approximately 750 μm through live tissues without invasive surgery and at a depth of approximately 2 mm in glycerol-cleared brain tissues. Near-infrared II light-sheet microscopy in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T-cell motion in tumor microcirculation and mapping out programmed-death ligand 1 and programmed cell death protein 1 in tumors with cellular resolution. Three-dimensional imaging through the intact mouse head resolved vascular channels between the skull and brain cortex, and allowed monitoring of recruitment of macrophages and microglia to the traumatic brain injury site.
Persistent Identifierhttp://hdl.handle.net/10722/325433
ISSN
2023 Impact Factor: 36.1
2023 SCImago Journal Rankings: 14.796
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Feifei-
dc.contributor.authorWan, Hao-
dc.contributor.authorMa, Zhuoran-
dc.contributor.authorZhong, Yeteng-
dc.contributor.authorSun, Qinchao-
dc.contributor.authorTian, Ye-
dc.contributor.authorQu, Liangqiong-
dc.contributor.authorDu, Haotian-
dc.contributor.authorZhang, Mingxi-
dc.contributor.authorLi, Lulin-
dc.contributor.authorMa, Huilong-
dc.contributor.authorLuo, Jian-
dc.contributor.authorLiang, Yongye-
dc.contributor.authorLi, Wen Jung-
dc.contributor.authorHong, Guosong-
dc.contributor.authorLiu, Lianqing-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-02-27T07:33:15Z-
dc.date.available2023-02-27T07:33:15Z-
dc.date.issued2019-
dc.identifier.citationNature Methods, 2019, v. 16, n. 6, p. 545-552-
dc.identifier.issn1548-7091-
dc.identifier.urihttp://hdl.handle.net/10722/325433-
dc.description.abstractNon-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000–1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,700 nm, respectively, for optical sectioning at a penetration depth of approximately 750 μm through live tissues without invasive surgery and at a depth of approximately 2 mm in glycerol-cleared brain tissues. Near-infrared II light-sheet microscopy in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T-cell motion in tumor microcirculation and mapping out programmed-death ligand 1 and programmed cell death protein 1 in tumors with cellular resolution. Three-dimensional imaging through the intact mouse head resolved vascular channels between the skull and brain cortex, and allowed monitoring of recruitment of macrophages and microglia to the traumatic brain injury site.-
dc.languageeng-
dc.relation.ispartofNature Methods-
dc.titleLight-sheet microscopy in the near-infrared II window-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/s41592-019-0398-7-
dc.identifier.pmid31086342-
dc.identifier.scopuseid_2-s2.0-85065791421-
dc.identifier.volume16-
dc.identifier.issue6-
dc.identifier.spage545-
dc.identifier.epage552-
dc.identifier.eissn1548-7105-
dc.identifier.isiWOS:000469455200024-

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