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- Publisher Website: 10.3389/fchem.2019.00844
- Scopus: eid_2-s2.0-85077242805
- WOS: WOS:000504103400001
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Article: Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets
Title | Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets |
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Authors | |
Keywords | fluorescent detection sensor single nucleotide polymorphisms ternary chalcogenide nanosheets two-dimensional nanomaterials |
Issue Date | 2019 |
Citation | Frontiers in Chemistry, 2019, v. 7, article no. 844 How to Cite? |
Abstract | Herein, we report that the ternary chalcogenide nanosheet exhibits different affinity toward oligonucleotides with different lengths and efficiently quenches the fluorescence of dye-labeled DNA probes. Based on these findings, as a proof-of-concept application, the ternary chalcogenide nanosheet is used as a target cyclic amplification biosensor, showing high specificity in discriminating single-base mismatch. This simple strategy is fast and sensitive for the single nucleotide polymorphism detection. Ultralow detection limit of unlabeled target (250 fM) and high discrimination ratio (5%) in the mixture of perfect match (mutant-type) and single-base mismatch (wild-type) target are achieved. This sensing method is extensively compatible for the single nucleotide polymorphism detection in clinical samples, making it a promising tool for the mutation-based clinical diagnostic and genomic research. |
Persistent Identifier | http://hdl.handle.net/10722/329597 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Hu, Yanling | - |
dc.contributor.author | Tan, Chaoliang | - |
dc.contributor.author | Lin, Xin | - |
dc.contributor.author | Lai, Zhuangchai | - |
dc.contributor.author | Zhang, Xiao | - |
dc.contributor.author | Lu, Qipeng | - |
dc.contributor.author | Feng, Ning | - |
dc.contributor.author | Yang, Dongliang | - |
dc.contributor.author | Weng, Lixing | - |
dc.date.accessioned | 2023-08-09T03:33:56Z | - |
dc.date.available | 2023-08-09T03:33:56Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Frontiers in Chemistry, 2019, v. 7, article no. 844 | - |
dc.identifier.uri | http://hdl.handle.net/10722/329597 | - |
dc.description.abstract | Herein, we report that the ternary chalcogenide nanosheet exhibits different affinity toward oligonucleotides with different lengths and efficiently quenches the fluorescence of dye-labeled DNA probes. Based on these findings, as a proof-of-concept application, the ternary chalcogenide nanosheet is used as a target cyclic amplification biosensor, showing high specificity in discriminating single-base mismatch. This simple strategy is fast and sensitive for the single nucleotide polymorphism detection. Ultralow detection limit of unlabeled target (250 fM) and high discrimination ratio (5%) in the mixture of perfect match (mutant-type) and single-base mismatch (wild-type) target are achieved. This sensing method is extensively compatible for the single nucleotide polymorphism detection in clinical samples, making it a promising tool for the mutation-based clinical diagnostic and genomic research. | - |
dc.language | eng | - |
dc.relation.ispartof | Frontiers in Chemistry | - |
dc.subject | fluorescent detection | - |
dc.subject | sensor | - |
dc.subject | single nucleotide polymorphisms | - |
dc.subject | ternary chalcogenide nanosheets | - |
dc.subject | two-dimensional nanomaterials | - |
dc.title | Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3389/fchem.2019.00844 | - |
dc.identifier.scopus | eid_2-s2.0-85077242805 | - |
dc.identifier.volume | 7 | - |
dc.identifier.spage | article no. 844 | - |
dc.identifier.epage | article no. 844 | - |
dc.identifier.eissn | 2296-2646 | - |
dc.identifier.isi | WOS:000504103400001 | - |