File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1038/s41522-023-00447-8
- Scopus: eid_2-s2.0-85174226664
- WOS: WOS:001084847400001
Supplementary
- Citations:
- Appears in Collections:
Article: Native microbiome dominates over host factors in shaping the probiotic genetic evolution in the gut
Title | Native microbiome dominates over host factors in shaping the probiotic genetic evolution in the gut |
---|---|
Authors | |
Issue Date | 1-Dec-2023 |
Publisher | Nature Research |
Citation | npj Biofilms and Microbiomes, 2023, v. 9, n. 1 How to Cite? |
Abstract | Probiotics often acquire potentially adaptive mutations in vivo, gaining new functional traits through gut selection. While both the host and microbiome can contribute to probiotics’ genetic evolution, separating the microbiome and the host’s contribution to such selective pressures remains challenging. Here, we introduced germ-free (GF) and specific pathogen-free (SPF) mouse models to track how probiotic strains, i.e., Lactiplantibacillus plantarum HNU082 (Lp082) and Bifidobacterium animalis subsp. lactis V9 (BV9), genetically evolved under selection pressures derived from host factors alone and both host and microbial ecological factors. Notably, compared to the genome of a probiotic strain before consumption, the host only elicited 99.75%). For a given probiotic, functional genes occurring in potentially adaptive mutations induced by hosts (GF mice) were all shared with those found in mutants of SPF mice. Collectively, the native microbiome consistently drove a more rapid and divergent genetic evolution of probiotic strains in seven days of colonization than host factors did. Our study further laid a theoretical foundation for genetically engineering probiotics for better gut adaptation through in vitro artificial gut ecosystems without the selection pressures derived from host factors. |
Persistent Identifier | http://hdl.handle.net/10722/337449 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiang, Shuaiming | - |
dc.contributor.author | Zhang, Chengcheng | - |
dc.contributor.author | Han, Zhe | - |
dc.contributor.author | Ma, Wenyao | - |
dc.contributor.author | Wang, Shunhe | - |
dc.contributor.author | Huo, Dongxue | - |
dc.contributor.author | Cui, Weipeng | - |
dc.contributor.author | Zhai, Qixiao | - |
dc.contributor.author | Huang, Shi | - |
dc.contributor.author | Zhang, Jiachao | - |
dc.date.accessioned | 2024-03-11T10:20:57Z | - |
dc.date.available | 2024-03-11T10:20:57Z | - |
dc.date.issued | 2023-12-01 | - |
dc.identifier.citation | npj Biofilms and Microbiomes, 2023, v. 9, n. 1 | - |
dc.identifier.uri | http://hdl.handle.net/10722/337449 | - |
dc.description.abstract | <p>Probiotics often acquire potentially adaptive mutations in vivo, gaining new functional traits through gut selection. While both the host and microbiome can contribute to probiotics’ genetic evolution, separating the microbiome and the host’s contribution to such selective pressures remains challenging. Here, we introduced germ-free (GF) and specific pathogen-free (SPF) mouse models to track how probiotic strains, i.e., Lactiplantibacillus plantarum HNU082 (Lp082) and Bifidobacterium animalis subsp. lactis V9 (BV9), genetically evolved under selection pressures derived from host factors alone and both host and microbial ecological factors. Notably, compared to the genome of a probiotic strain before consumption, the host only elicited 99.75%). For a given probiotic, functional genes occurring in potentially adaptive mutations induced by hosts (GF mice) were all shared with those found in mutants of SPF mice. Collectively, the native microbiome consistently drove a more rapid and divergent genetic evolution of probiotic strains in seven days of colonization than host factors did. Our study further laid a theoretical foundation for genetically engineering probiotics for better gut adaptation through in vitro artificial gut ecosystems without the selection pressures derived from host factors. <br></p> | - |
dc.language | eng | - |
dc.publisher | Nature Research | - |
dc.relation.ispartof | npj Biofilms and Microbiomes | - |
dc.title | Native microbiome dominates over host factors in shaping the probiotic genetic evolution in the gut | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41522-023-00447-8 | - |
dc.identifier.scopus | eid_2-s2.0-85174226664 | - |
dc.identifier.volume | 9 | - |
dc.identifier.issue | 1 | - |
dc.identifier.eissn | 2055-5008 | - |
dc.identifier.isi | WOS:001084847400001 | - |
dc.identifier.issnl | 2055-5008 | - |