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Article: The inhibitory effect of Gleditsia sinensis on cyclooxygenase-2 expression in human esophageal squamous cell carcinoma
Title | The inhibitory effect of Gleditsia sinensis on cyclooxygenase-2 expression in human esophageal squamous cell carcinoma | ||||||||
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Authors | |||||||||
Keywords | Anti-proliferation Cyclooxygenase-2 Esophaegal squamous cell carcinoma Gene over-expression | ||||||||
Issue Date | 2009 | ||||||||
Publisher | Demetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm | ||||||||
Citation | International Journal Of Molecular Medicine, 2009, v. 23 n. 1, p. 121-129 How to Cite? | ||||||||
Abstract | The anti-cancer effects of the anomalous fruit extract of Gleditsia sinensis (GSE) attributed to its apoptotic activity, telomerase inhibition and anti-angiogenesis in a panel of solid and non-solid tumor cell lines including esophageal squamous cell carcinoma (ESCC) have been intensively investigated by us in previous studies. Cyclooxygenase-2 (COX-2) has been well described as another promising target of cancer therapy for ESCC, and novel therapeutic agents are still being sought which target COX-2 expression. However, the anti-cancer effect of GSE through the suppression of COX-2 expression has not been previously investigated. In the present study, the anti-cancer effects of GSE on eight ESCC cell lines (KYSE 30, KYSE 150, KYSE 450, KYSE 510, KYSE 520, HKESC-3, HKESC-4 and SLMT-1) of Chinese and Japanese origins were first studied by MTS cytotoxicity assays. The effects of GSE on COX-2 expression levels and on the housekeeping form COX-1 were also investigated by multiplex RT-PCR analysis. Moreover, the anti-proliferative effect of GSE on KYSE 510 was also studied by anchorage-independent clonogenicity assay in soft agar. The results showed that GSE induced a dose- and time-dependent cytotoxicity on all of the eight ESCC cell lines and caused positive anti-proliferative action on KYSE 510 in the anchorage-independent clonogenicity assay, suggesting that GSE suppressed the in vitro growth of the ESCC cell lines. More importantly, the MRNA expression levels of COX-2, but not COX-1, in all of the ESCC cell lines were suppressed by GSE in a dose-dependent fashion. The overall results of the present study show that the anti-cancer effect of GSE on the ESCC cell lines is associated with the suppression of COX-2 expression, but not COX-1. Our findings also open a new chapter for the future advancement of GSE as a novel anticancer agent or as an adjuvant of traditional cancer treatments. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/60583 | ||||||||
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.167 | ||||||||
ISI Accession Number ID |
Funding Information: We acknowledge the donation of Mr. K.C. Lo for setting up the Lo Ka Chung Centre for Natural Anti-Cancer Drug Development and for supporting the research described in this report. The present study was also supported by the PolyU General Research Fund (grant no. G-U255), PolyU Niche Area Fund (grant no. BB65) for the project 'Novel Anti-Cancer Small Molecule Drug R&D' and the Niche Area Fund for the development of traditional Chinese medicine (grant no. BB8P). This research study is also part of the M.Phil. study of K.C. Pak. | ||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pak, KC | en_HK |
dc.contributor.author | Lam, KY | en_HK |
dc.contributor.author | Law, S | en_HK |
dc.contributor.author | Tang, JCO | en_HK |
dc.date.accessioned | 2010-05-31T04:14:09Z | - |
dc.date.available | 2010-05-31T04:14:09Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | International Journal Of Molecular Medicine, 2009, v. 23 n. 1, p. 121-129 | en_HK |
dc.identifier.issn | 1107-3756 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/60583 | - |
dc.description.abstract | The anti-cancer effects of the anomalous fruit extract of Gleditsia sinensis (GSE) attributed to its apoptotic activity, telomerase inhibition and anti-angiogenesis in a panel of solid and non-solid tumor cell lines including esophageal squamous cell carcinoma (ESCC) have been intensively investigated by us in previous studies. Cyclooxygenase-2 (COX-2) has been well described as another promising target of cancer therapy for ESCC, and novel therapeutic agents are still being sought which target COX-2 expression. However, the anti-cancer effect of GSE through the suppression of COX-2 expression has not been previously investigated. In the present study, the anti-cancer effects of GSE on eight ESCC cell lines (KYSE 30, KYSE 150, KYSE 450, KYSE 510, KYSE 520, HKESC-3, HKESC-4 and SLMT-1) of Chinese and Japanese origins were first studied by MTS cytotoxicity assays. The effects of GSE on COX-2 expression levels and on the housekeeping form COX-1 were also investigated by multiplex RT-PCR analysis. Moreover, the anti-proliferative effect of GSE on KYSE 510 was also studied by anchorage-independent clonogenicity assay in soft agar. The results showed that GSE induced a dose- and time-dependent cytotoxicity on all of the eight ESCC cell lines and caused positive anti-proliferative action on KYSE 510 in the anchorage-independent clonogenicity assay, suggesting that GSE suppressed the in vitro growth of the ESCC cell lines. More importantly, the MRNA expression levels of COX-2, but not COX-1, in all of the ESCC cell lines were suppressed by GSE in a dose-dependent fashion. The overall results of the present study show that the anti-cancer effect of GSE on the ESCC cell lines is associated with the suppression of COX-2 expression, but not COX-1. Our findings also open a new chapter for the future advancement of GSE as a novel anticancer agent or as an adjuvant of traditional cancer treatments. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Demetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm | en_HK |
dc.relation.ispartof | International Journal of Molecular Medicine | en_HK |
dc.subject | Anti-proliferation | en_HK |
dc.subject | Cyclooxygenase-2 | en_HK |
dc.subject | Esophaegal squamous cell carcinoma | en_HK |
dc.subject | Gene over-expression | en_HK |
dc.title | The inhibitory effect of Gleditsia sinensis on cyclooxygenase-2 expression in human esophageal squamous cell carcinoma | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1107-3756&volume=23&issue=1&spage=121&epage=129&date=2009&atitle=The+inhibitory+effect+of+Gleditsia+sinensis+on+cyclooxygenase-2+expression+in+human+esophageal+squamous+cell+carcinoma | en_HK |
dc.identifier.email | Law, S: slaw@hku.hk | en_HK |
dc.identifier.authority | Law, S=rp00437 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3892/ijmm_00000109 | en_HK |
dc.identifier.scopus | eid_2-s2.0-61749090021 | en_HK |
dc.identifier.hkuros | 154280 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-61749090021&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 23 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 121 | en_HK |
dc.identifier.epage | 129 | en_HK |
dc.identifier.isi | WOS:000262014000015 | - |
dc.publisher.place | Greece | en_HK |
dc.identifier.scopusauthorid | Pak, KC=7102341256 | en_HK |
dc.identifier.scopusauthorid | Lam, KY=7403657165 | en_HK |
dc.identifier.scopusauthorid | Law, S=7202241293 | en_HK |
dc.identifier.scopusauthorid | Tang, JCO=14056850300 | en_HK |
dc.identifier.issnl | 1107-3756 | - |