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Article: Promoter hypermethylation of high-in-normal 1 gene in primary nasopharyngeal carcinoma
Title | Promoter hypermethylation of high-in-normal 1 gene in primary nasopharyngeal carcinoma |
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Authors | |
Issue Date | 2003 |
Publisher | American Association for Cancer Research. |
Citation | Clinical Cancer Research, 2003, v. 9 n. 8, p. 3042-3046 How to Cite? |
Abstract | Purpose: The methylation of high-in-normal-1 (HIN-1) gene promoter in undifferentiated nasopharyngeal carcinoma (NPC) is studied. Experimental design: The methylation status of HIN-1 in NPC cell lines, primary NPC, paired nasopharyngeal swabs, paired throat-rinsing fluid, and paired peripheral blood was assessed by methylation-specific PCR assay. The relationship between HIN-1 promoter methylation and transcription in NPC cell lines was evaluated by reverse transcription-PCR and demethylation agent treatment (5-aza-2-deoxycytidine). Results: Hypermethylated promoter was observed in five of five (100%) NPC cell lines and not found in three normal nasopharyngeal outgrowths, two tonsil epithelial cell cultures, and two skin fibroblast cultures. Reverse transcription-PCR assay indicated that HIN-1 transcription was significantly down-regulated in the NPC cell line with promoter methylation. Treatment with demethylation agent, 5-aza-2-deoxycytidine, restored HIN-1 transcription in the NPC cell line. Methylated HIN-1 promoter was found in 36 of 47 (77%) primary NPC tumors and not found in the normal nasopharyngeal biopsies. Methylated HIN-1 promoter was detected in 12 of 26 (46%) nasopharyngeal swabs, 5 of 26 (19%) throat-rinsing fluids, 2 of 11 (18%) plasmas, and 5 of 11 (46%) buffy coats of peripheral blood of the NPC patients but was not detectable in all normal controls. Conclusion: HIN-1 promoter hypermethylation is common in NPC. Methylated promoter DNA in nasopharyngeal swab, throat-rinsing fluid, and peripheral blood might be potentially useful as tumor marker for screening of NPC. |
Persistent Identifier | http://hdl.handle.net/10722/67934 |
ISSN | 2023 Impact Factor: 10.0 2023 SCImago Journal Rankings: 4.623 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, TS | en_HK |
dc.contributor.author | Kwong, DLW | en_HK |
dc.contributor.author | Sham, JST | en_HK |
dc.contributor.author | Tsao, SW | en_HK |
dc.contributor.author | Wei, WI | en_HK |
dc.contributor.author | Kwong, YL | en_HK |
dc.contributor.author | Yuen, APW | en_HK |
dc.date.accessioned | 2010-09-06T05:59:36Z | - |
dc.date.available | 2010-09-06T05:59:36Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Clinical Cancer Research, 2003, v. 9 n. 8, p. 3042-3046 | en_HK |
dc.identifier.issn | 1078-0432 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67934 | - |
dc.description.abstract | Purpose: The methylation of high-in-normal-1 (HIN-1) gene promoter in undifferentiated nasopharyngeal carcinoma (NPC) is studied. Experimental design: The methylation status of HIN-1 in NPC cell lines, primary NPC, paired nasopharyngeal swabs, paired throat-rinsing fluid, and paired peripheral blood was assessed by methylation-specific PCR assay. The relationship between HIN-1 promoter methylation and transcription in NPC cell lines was evaluated by reverse transcription-PCR and demethylation agent treatment (5-aza-2-deoxycytidine). Results: Hypermethylated promoter was observed in five of five (100%) NPC cell lines and not found in three normal nasopharyngeal outgrowths, two tonsil epithelial cell cultures, and two skin fibroblast cultures. Reverse transcription-PCR assay indicated that HIN-1 transcription was significantly down-regulated in the NPC cell line with promoter methylation. Treatment with demethylation agent, 5-aza-2-deoxycytidine, restored HIN-1 transcription in the NPC cell line. Methylated HIN-1 promoter was found in 36 of 47 (77%) primary NPC tumors and not found in the normal nasopharyngeal biopsies. Methylated HIN-1 promoter was detected in 12 of 26 (46%) nasopharyngeal swabs, 5 of 26 (19%) throat-rinsing fluids, 2 of 11 (18%) plasmas, and 5 of 11 (46%) buffy coats of peripheral blood of the NPC patients but was not detectable in all normal controls. Conclusion: HIN-1 promoter hypermethylation is common in NPC. Methylated promoter DNA in nasopharyngeal swab, throat-rinsing fluid, and peripheral blood might be potentially useful as tumor marker for screening of NPC. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Association for Cancer Research. | en_HK |
dc.relation.ispartof | Clinical Cancer Research | en_HK |
dc.subject.mesh | Adult | en_HK |
dc.subject.mesh | Aged | en_HK |
dc.subject.mesh | Carcinoma - blood - diagnosis - genetics | en_HK |
dc.subject.mesh | Cell Differentiation | en_HK |
dc.subject.mesh | Cytokines - blood - genetics - metabolism | en_HK |
dc.subject.mesh | DNA Methylation | en_HK |
dc.subject.mesh | Dose-Response Relationship, Drug | en_HK |
dc.subject.mesh | Female | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Middle Aged | en_HK |
dc.subject.mesh | Nasopharyngeal Neoplasms - blood - diagnosis - genetics | en_HK |
dc.subject.mesh | Polymerase Chain Reaction | en_HK |
dc.subject.mesh | Promoter Regions, Genetic | en_HK |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_HK |
dc.subject.mesh | Tumor Suppressor Proteins - blood - genetics - metabolism | en_HK |
dc.title | Promoter hypermethylation of high-in-normal 1 gene in primary nasopharyngeal carcinoma | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1078-0432&volume=9&spage=3042&epage=3046&date=2003&atitle=Promoter+Hypermethylation+Of+High-in-normal+1+Gene+In+Primary+Nasopharyngeal+Carcinoma | en_HK |
dc.identifier.email | Wong, TS: thiansze@graduate.hku.hk | en_HK |
dc.identifier.email | Kwong, DLW: dlwkwong@hku.hk | en_HK |
dc.identifier.email | Tsao, SW: gswtsao@hkucc.hku.hk | en_HK |
dc.identifier.email | Wei, WI: hrmswwi@hku.hk | en_HK |
dc.identifier.email | Kwong, YL: ylkwong@hku.hk | en_HK |
dc.identifier.authority | Wong, TS=rp00478 | en_HK |
dc.identifier.authority | Kwong, DLW=rp00414 | en_HK |
dc.identifier.authority | Tsao, SW=rp00399 | en_HK |
dc.identifier.authority | Wei, WI=rp00323 | en_HK |
dc.identifier.authority | Kwong, YL=rp00358 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.pmid | 12912954 | - |
dc.identifier.scopus | eid_2-s2.0-0042025015 | en_HK |
dc.identifier.hkuros | 82038 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0042025015&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 9 | en_HK |
dc.identifier.issue | 8 | en_HK |
dc.identifier.spage | 3042 | en_HK |
dc.identifier.epage | 3046 | en_HK |
dc.identifier.isi | WOS:000184680200025 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Wong, TS=7403531328 | en_HK |
dc.identifier.scopusauthorid | Kwong, DLW=15744231600 | en_HK |
dc.identifier.scopusauthorid | Sham, JST=24472255400 | en_HK |
dc.identifier.scopusauthorid | Tsao, SW=7102813116 | en_HK |
dc.identifier.scopusauthorid | Wei, WI=7403321552 | en_HK |
dc.identifier.scopusauthorid | Kwong, YL=7102818954 | en_HK |
dc.identifier.scopusauthorid | Yuen, APW=7006290111 | en_HK |
dc.identifier.issnl | 1078-0432 | - |