Optimization of culture medium for growth of Haematococcus pluvialis

Abstract

A central composite rotatable design was used to examine the effects of five components of the medium on the growth of Haematococcus pluvialis in batch culture. The medium components considered were: sodium acetate, potassium nitrate, major elements, trace elements and vitamins. Within the range of the concentrations tested, a moderate concentration of the major elements significantly enhanced algal growth, both in terms of specific growth rate and cell dry weight, whereas the vitamins had no significant effect. Based on the response surface contour plots and the results of numerical analyses, the optimal nutrient concentrations for growth in terms of specific growth rate were 0.51 g L-1 sodium acetate, 0.25 g L-1 potassium nitrate, 0.63 mL L-1 of the major element stock solution and 0.2 mL L-1 of the trace element stock solution. The optimal nutrient concentrations for biomass production were 1.64 g L-1 sodium acetate, 0.37 g L-1 potassium nitrate, 2.52 mL L-1 of the major element stock solution and 0.03 mL L-1 of the trace element stock solution.