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- Publisher Website: 10.1053/jlts.2003.50066
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- PMID: 12682897
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Article: Intragraft gene expression profiles by cDNA microarray in small-for-size liver grafts
Title | Intragraft gene expression profiles by cDNA microarray in small-for-size liver grafts |
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Authors | |
Issue Date | 2003 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021 |
Citation | Liver Transplantation, 2003, v. 9 n. 4, p. 425-432 How to Cite? |
Abstract | The aim of this study is to identify the molecular mechanism of small-for-size graft injury through large-scale expression measurement of intragraft gene profile by carrier DNA (cDNA) microarray screening in liver transplantation. The studies compared 1,081 intragraft genes expression profiles using cDNA microarray of small-for-size grafts (<30% of recipient liver weight) with those of whole grafts (control group) 1, 3, and 24 hours after reperfusion in a rat liver transplantation model. Intragraft gene expression was detected by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR). Hepatic ultrastructural features were shown by electron microscopy. In the small-for-size grafts, by cDNA microarray study, the vasoconstriction genes were found up-regulated together with adhesion molecules at 1 hour after reperfusion. Three and 24 hours after reperfusion, the vasopressin genes were found up-regulated together with adhesion molecules, inflammatory mediators and cell death signals, accompanied with down-regulation of the genes related to energy metabolism. By quantitative RT-PCR, intragraft messenger RNA (mRNA) expression of endothelin-1 (ET-1) and endothelin-1 receptor A (ETA) was up-regulated during the first 24 hours after reperfusion accompanied with down-regulation of heme oxygenase-1 (HO-1). The intragraft mRNA and plasma levels of inflammatory cytokines (interleukin [IL]-6, IL-15, tumor necrosis factor [TNF]-α) also were overexpressed during the first 24 hours after reperfusion. Sinusoidal congestion and disruption were found accompanied with mitochondrial swelling during the first 24 hours after reperfusion. The up-regulation of intragraft vasoconstriction genes accompanied by early overexpression of adhesion molecules and apoptotic signals, as well as down-regulation of HO-1 in small-for-size grafts may be related to sinusoidal injury leading to graft damage in liver transplantation. |
Persistent Identifier | http://hdl.handle.net/10722/88467 |
ISSN | 2023 Impact Factor: 4.7 2023 SCImago Journal Rankings: 1.700 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Man, K | en_HK |
dc.contributor.author | Lo, CM | en_HK |
dc.contributor.author | Lee, TKW | en_HK |
dc.contributor.author | Li, XL | en_HK |
dc.contributor.author | Ng, IOL | en_HK |
dc.contributor.author | Fan, ST | en_HK |
dc.date.accessioned | 2010-09-06T09:43:46Z | - |
dc.date.available | 2010-09-06T09:43:46Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Liver Transplantation, 2003, v. 9 n. 4, p. 425-432 | en_HK |
dc.identifier.issn | 1527-6465 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/88467 | - |
dc.description.abstract | The aim of this study is to identify the molecular mechanism of small-for-size graft injury through large-scale expression measurement of intragraft gene profile by carrier DNA (cDNA) microarray screening in liver transplantation. The studies compared 1,081 intragraft genes expression profiles using cDNA microarray of small-for-size grafts (<30% of recipient liver weight) with those of whole grafts (control group) 1, 3, and 24 hours after reperfusion in a rat liver transplantation model. Intragraft gene expression was detected by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR). Hepatic ultrastructural features were shown by electron microscopy. In the small-for-size grafts, by cDNA microarray study, the vasoconstriction genes were found up-regulated together with adhesion molecules at 1 hour after reperfusion. Three and 24 hours after reperfusion, the vasopressin genes were found up-regulated together with adhesion molecules, inflammatory mediators and cell death signals, accompanied with down-regulation of the genes related to energy metabolism. By quantitative RT-PCR, intragraft messenger RNA (mRNA) expression of endothelin-1 (ET-1) and endothelin-1 receptor A (ETA) was up-regulated during the first 24 hours after reperfusion accompanied with down-regulation of heme oxygenase-1 (HO-1). The intragraft mRNA and plasma levels of inflammatory cytokines (interleukin [IL]-6, IL-15, tumor necrosis factor [TNF]-α) also were overexpressed during the first 24 hours after reperfusion. Sinusoidal congestion and disruption were found accompanied with mitochondrial swelling during the first 24 hours after reperfusion. The up-regulation of intragraft vasoconstriction genes accompanied by early overexpression of adhesion molecules and apoptotic signals, as well as down-regulation of HO-1 in small-for-size grafts may be related to sinusoidal injury leading to graft damage in liver transplantation. | en_HK |
dc.language | eng | en_HK |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021 | en_HK |
dc.relation.ispartof | Liver Transplantation | en_HK |
dc.rights | Liver Transplantation. Copyright © John Wiley & Sons, Inc. | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Computer Systems | en_HK |
dc.subject.mesh | Cytokines - blood - genetics | en_HK |
dc.subject.mesh | Down-Regulation | en_HK |
dc.subject.mesh | Endothelin-1 - blood - genetics | en_HK |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | en_HK |
dc.subject.mesh | Gene Expression Profiling | en_HK |
dc.subject.mesh | Heme Oxygenase (Decyclizing) - genetics | en_HK |
dc.subject.mesh | Heme Oxygenase-1 | en_HK |
dc.subject.mesh | Liver - metabolism - ultrastructure | en_HK |
dc.subject.mesh | Liver Transplantation - methods | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Microscopy, Electron | en_HK |
dc.subject.mesh | Oligonucleotide Array Sequence Analysis | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Rats, Sprague-Dawley | en_HK |
dc.subject.mesh | Receptors, Endothelin - genetics | en_HK |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_HK |
dc.title | Intragraft gene expression profiles by cDNA microarray in small-for-size liver grafts | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1527-6465&volume=9&spage=425&epage=432&date=2003&atitle=Intragraft+gene+expression+profiles+by+cDNA+microarray+in+small-for-size+liver+grafts | en_HK |
dc.identifier.email | Man, K: kwanman@hkucc.hku.hk | en_HK |
dc.identifier.email | Lo, CM: chungmlo@hkucc.hku.hk | en_HK |
dc.identifier.email | Lee, TKW: tkwlee@hkucc.hku.hk | en_HK |
dc.identifier.email | Ng, IOL: iolng@hkucc.hku.hk | en_HK |
dc.identifier.email | Fan, ST: stfan@hku.hk | en_HK |
dc.identifier.authority | Man, K=rp00417 | en_HK |
dc.identifier.authority | Lo, CM=rp00412 | en_HK |
dc.identifier.authority | Lee, TKW=rp00447 | en_HK |
dc.identifier.authority | Ng, IOL=rp00335 | en_HK |
dc.identifier.authority | Fan, ST=rp00355 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1053/jlts.2003.50066 | en_HK |
dc.identifier.pmid | 12682897 | - |
dc.identifier.scopus | eid_2-s2.0-0037387674 | en_HK |
dc.identifier.hkuros | 76920 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037387674&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 9 | en_HK |
dc.identifier.issue | 4 | en_HK |
dc.identifier.spage | 425 | en_HK |
dc.identifier.epage | 432 | en_HK |
dc.identifier.isi | WOS:000182009300017 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Man, K=7101754072 | en_HK |
dc.identifier.scopusauthorid | Lo, CM=7401771672 | en_HK |
dc.identifier.scopusauthorid | Lee, TKW=7501439435 | en_HK |
dc.identifier.scopusauthorid | Li, XL=13008588500 | en_HK |
dc.identifier.scopusauthorid | Ng, IOL=7102753722 | en_HK |
dc.identifier.scopusauthorid | Fan, ST=7402678224 | en_HK |
dc.identifier.issnl | 1527-6465 | - |