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Article: Comparison of two laboratory extraction techniques for the detection of Epstein–Barr virus in the saliva of nasopharyngeal carcinoma patients

TitleComparison of two laboratory extraction techniques for the detection of Epstein–Barr virus in the saliva of nasopharyngeal carcinoma patients
Authors
KeywordsDNA
Epstein-Barr virus
Polymerase chain reaction
Saliva
Nasopharyngeal carcinoma
Issue Date2014
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=2041-1618&site=1
Citation
Journal of Investigative and Clinical Dentistry , 2014, v. 5 n. 2, p. 104-108 How to Cite?
Abstract© 2013 Wiley Publishing Asia Pty Ltd. AIM: The aim of the present study was to compare the effectiveness of DNA extraction using an extraction kit against the standard boiling technique for the detection of Epstein-Barr virus (EBV) DNA in nasopharyngeal carcinoma (NPC) patients.METHODS: Stimulated whole saliva samples from newly-diagnosed NPC patients were collected. EBV DNA was extracted by both techniques (n = 23) followed by quantitative real-time polymerase chain reaction (PCR) using the primer/probe set for BALF5.RESULTS: The results of the quantitative real-time PCR were reproducible in both groups. The two techniques were moderately correlated (r = 0.67, P < 0.05), and the degree of agreement was good. However, the mean EBV DNA level in the boiling group (3.02 ± 8.67 × 10(6) copies/μL) was significantly higher than the extraction kit group (1.15 ± 2.66 × 10(6) copies/μL) (P < 0.05). The EBV DNA level was higher in patients at an advanced overall stage (P = 0.05).CONCLUSION: The results of the present study showed that the performance of the extraction kit was not superior to the simple boiling technique for the detection of salivary EBV DNA in NPC patients using real-time PCR. The salivary EBV DNA level in patients at an advanced overall stage appeared to be higher than in patients at an early stage.
Persistent Identifierhttp://hdl.handle.net/10722/202475
ISSN
2015 SCImago Journal Rankings: 0.268
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShan, J-
dc.contributor.authorPow, EHN-
dc.contributor.authorTsang, PCS-
dc.contributor.authorPerera, RAPM-
dc.contributor.authorKwong, DLW-
dc.date.accessioned2014-09-19T07:50:44Z-
dc.date.available2014-09-19T07:50:44Z-
dc.date.issued2014-
dc.identifier.citationJournal of Investigative and Clinical Dentistry , 2014, v. 5 n. 2, p. 104-108-
dc.identifier.issn2041-1618-
dc.identifier.urihttp://hdl.handle.net/10722/202475-
dc.description.abstract© 2013 Wiley Publishing Asia Pty Ltd. AIM: The aim of the present study was to compare the effectiveness of DNA extraction using an extraction kit against the standard boiling technique for the detection of Epstein-Barr virus (EBV) DNA in nasopharyngeal carcinoma (NPC) patients.METHODS: Stimulated whole saliva samples from newly-diagnosed NPC patients were collected. EBV DNA was extracted by both techniques (n = 23) followed by quantitative real-time polymerase chain reaction (PCR) using the primer/probe set for BALF5.RESULTS: The results of the quantitative real-time PCR were reproducible in both groups. The two techniques were moderately correlated (r = 0.67, P < 0.05), and the degree of agreement was good. However, the mean EBV DNA level in the boiling group (3.02 ± 8.67 × 10(6) copies/μL) was significantly higher than the extraction kit group (1.15 ± 2.66 × 10(6) copies/μL) (P < 0.05). The EBV DNA level was higher in patients at an advanced overall stage (P = 0.05).CONCLUSION: The results of the present study showed that the performance of the extraction kit was not superior to the simple boiling technique for the detection of salivary EBV DNA in NPC patients using real-time PCR. The salivary EBV DNA level in patients at an advanced overall stage appeared to be higher than in patients at an early stage.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=2041-1618&site=1-
dc.relation.ispartofJournal of Investigative and Clinical Dentistry-
dc.subjectDNA-
dc.subjectEpstein-Barr virus-
dc.subjectPolymerase chain reaction-
dc.subjectSaliva-
dc.subjectNasopharyngeal carcinoma-
dc.titleComparison of two laboratory extraction techniques for the detection of Epstein–Barr virus in the saliva of nasopharyngeal carcinoma patients-
dc.typeArticle-
dc.identifier.emailPow, EHN: ehnpow@hku.hk-
dc.identifier.emailTsang, PCS: csptsang@hkucc.hku.hk-
dc.identifier.emailPerera, RAPM: mahenperera@hku.hk-
dc.identifier.emailKwong, DLW: dlwkwong@hku.hk-
dc.identifier.authorityPow, EHN=rp00030-
dc.identifier.authorityTsang, PCS=rp00026-
dc.identifier.authorityPerera, RAPM=rp02500-
dc.identifier.authorityKwong, DLW=rp00414-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/jicd.12078-
dc.identifier.pmid24574317-
dc.identifier.scopuseid_2-s2.0-84908099646-
dc.identifier.hkuros240098-
dc.identifier.hkuros229449-
dc.identifier.hkuros296995-
dc.identifier.volume5-
dc.identifier.issue2-
dc.identifier.spage104-
dc.identifier.epage108-
dc.identifier.isiWOS:000214115100004-
dc.publisher.placeUnited States-

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