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Conference Paper: Evolutionary changes of hepatitis B virus pre-S mutations prior to development of hepatocellular carcinoma
Title | Evolutionary changes of hepatitis B virus pre-S mutations prior to development of hepatocellular carcinoma |
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Authors | |
Keywords | Medical sciences Gastroenterology |
Issue Date | 2015 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep |
Citation | The 50th Annual Meeting of the European Association for the Study of the Liver (International Liver Congress™ 2015), Vienna, Austria, 22-26 April 2015. In Journal of Hepatology, 2015, v. 62 n. suppl. 2, p. S543, abstract no. P0604 How to Cite? |
Abstract | BACKGROUND AND AIMS: Hepatitis B virus (HBV) pre-S deletions/mutations have been associated with hepatocellular carcinoma (HCC). However, the evolutionary changes of pre-S mutations prior to HCC diagnosis remain unexplored. METHODS: HBV pre-S sequences were determined in 74 chronic hepatitis B (CHB) patients with HCC and 146 CHB patients who have been followed up for >3 years with no HCC (HCC-free). Samples collected at the following time points were studied: 1–3 years, 4–6 years, 7–9 years and ≥10 years prior to HCC development and at the same time points for the HCC-free group from last follow-up. HBV pre-S mutations were assessed by comparison with 54 wild-type reference sequences retrieved from the NCBI Genbank. RESULTS: Pre-S deletions were detected in 31.1%, 25%, 48.5%, and 42.8% of HCC patients at 1–3 years, 4–6 years, 7–9 years, and ≥10 years prior to HCC development, respectively. In the HCC-free group, the frequencies of pre-S deletions at the 4 corresponding time points were lower (9.6%, 7.9%, 8.8%, and 17.6%, respectively; with p values of <0.001, 0.036, <0.001, and 0.096, respectively). At 1–3 years prior to HCC development, higher frequencies of pre-S point mutations at 11 codons (codons 4, 27, 51, 54, 60, 62, 100, 125, 137, 166 and 167) were observed in the HCC patients (range: 14.9–75.7%) than in the HCC-free patients (range: 5.5–26%; all p < 0.05). Ten out of these 11 pre-S mutations (except codon 4, which emerged at 4–6 years before HCC) existed ≥10 years before HCC. Among these 11 codons, the number of mutated codons with differential distribution between HCC and HCC-free patients increased with time prior to HCC: a significantly higher frequency of mutations was detected in the HCC patients in 2/11 codons at ≥10 years, in 9/11 codons at 7–9 years, and in 10/11 codons at 4–6 years prior to HCC. The total number of HCC-associated pre-S point mutations increased with time prior to HCC and correlated with the time to HCC development (r = 0.151, p = 0.042), while no correlation was observed in the HCC-free group (r = 0.073, p = 0.398). Multivariable logistic regression analysis showed that pre-S deletions and point mutations at codons 27, 51 and 167 were independent factors associated with increased HCC risk (all p < 0.05). CONCLUSIONS: High prevalence and cumulative evolution of pre-S mutations preceding HCC development confirmed the carcinogenic role of pre-S mutations. The effect of individual pre-S mutations on HCC development warrants further studies. |
Description | Poster Presentation: no. P0604 This journal suppl. entitled: Abstracts of The International Liver Congress™ 2015 - 50 Annual meeting of the European Association for the Study of the Liver |
Persistent Identifier | http://hdl.handle.net/10722/214869 |
ISSN | 2023 Impact Factor: 26.8 2023 SCImago Journal Rankings: 9.857 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhang, A | - |
dc.contributor.author | Wong, DKH | - |
dc.contributor.author | Huang, FY | - |
dc.contributor.author | Seto, WK | - |
dc.contributor.author | Fung, J | - |
dc.contributor.author | Lai, CL | - |
dc.contributor.author | Yuen, MF | - |
dc.date.accessioned | 2015-08-21T12:00:03Z | - |
dc.date.available | 2015-08-21T12:00:03Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | The 50th Annual Meeting of the European Association for the Study of the Liver (International Liver Congress™ 2015), Vienna, Austria, 22-26 April 2015. In Journal of Hepatology, 2015, v. 62 n. suppl. 2, p. S543, abstract no. P0604 | - |
dc.identifier.issn | 0168-8278 | - |
dc.identifier.uri | http://hdl.handle.net/10722/214869 | - |
dc.description | Poster Presentation: no. P0604 | - |
dc.description | This journal suppl. entitled: Abstracts of The International Liver Congress™ 2015 - 50 Annual meeting of the European Association for the Study of the Liver | - |
dc.description.abstract | BACKGROUND AND AIMS: Hepatitis B virus (HBV) pre-S deletions/mutations have been associated with hepatocellular carcinoma (HCC). However, the evolutionary changes of pre-S mutations prior to HCC diagnosis remain unexplored. METHODS: HBV pre-S sequences were determined in 74 chronic hepatitis B (CHB) patients with HCC and 146 CHB patients who have been followed up for >3 years with no HCC (HCC-free). Samples collected at the following time points were studied: 1–3 years, 4–6 years, 7–9 years and ≥10 years prior to HCC development and at the same time points for the HCC-free group from last follow-up. HBV pre-S mutations were assessed by comparison with 54 wild-type reference sequences retrieved from the NCBI Genbank. RESULTS: Pre-S deletions were detected in 31.1%, 25%, 48.5%, and 42.8% of HCC patients at 1–3 years, 4–6 years, 7–9 years, and ≥10 years prior to HCC development, respectively. In the HCC-free group, the frequencies of pre-S deletions at the 4 corresponding time points were lower (9.6%, 7.9%, 8.8%, and 17.6%, respectively; with p values of <0.001, 0.036, <0.001, and 0.096, respectively). At 1–3 years prior to HCC development, higher frequencies of pre-S point mutations at 11 codons (codons 4, 27, 51, 54, 60, 62, 100, 125, 137, 166 and 167) were observed in the HCC patients (range: 14.9–75.7%) than in the HCC-free patients (range: 5.5–26%; all p < 0.05). Ten out of these 11 pre-S mutations (except codon 4, which emerged at 4–6 years before HCC) existed ≥10 years before HCC. Among these 11 codons, the number of mutated codons with differential distribution between HCC and HCC-free patients increased with time prior to HCC: a significantly higher frequency of mutations was detected in the HCC patients in 2/11 codons at ≥10 years, in 9/11 codons at 7–9 years, and in 10/11 codons at 4–6 years prior to HCC. The total number of HCC-associated pre-S point mutations increased with time prior to HCC and correlated with the time to HCC development (r = 0.151, p = 0.042), while no correlation was observed in the HCC-free group (r = 0.073, p = 0.398). Multivariable logistic regression analysis showed that pre-S deletions and point mutations at codons 27, 51 and 167 were independent factors associated with increased HCC risk (all p < 0.05). CONCLUSIONS: High prevalence and cumulative evolution of pre-S mutations preceding HCC development confirmed the carcinogenic role of pre-S mutations. The effect of individual pre-S mutations on HCC development warrants further studies. | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep | - |
dc.relation.ispartof | Journal of Hepatology | - |
dc.subject | Medical sciences | - |
dc.subject | Gastroenterology | - |
dc.title | Evolutionary changes of hepatitis B virus pre-S mutations prior to development of hepatocellular carcinoma | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Wong, DKH: danywong@hku.hk | - |
dc.identifier.email | Huang, FY: fungyu@hkucc.hku.hk | - |
dc.identifier.email | Seto, WK: wkseto@hku.hk | - |
dc.identifier.email | Fung, J: jfung@hkucc.hku.hk | - |
dc.identifier.email | Lai, CL: hrmelcl@hkucc.hku.hk | - |
dc.identifier.email | Yuen, MF: mfyuen@hku.hk | - |
dc.identifier.authority | Wong, DKH=rp00492 | - |
dc.identifier.authority | Seto, WK=rp01659 | - |
dc.identifier.authority | Fung, J=rp00518 | - |
dc.identifier.authority | Lai, CL=rp00314 | - |
dc.identifier.authority | Yuen, MF=rp00479 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S0168-8278(15)30810-2 | - |
dc.identifier.hkuros | 248020 | - |
dc.identifier.volume | 62 | - |
dc.identifier.issue | suppl. 2 | - |
dc.identifier.spage | S543, abstract no. P0604 | - |
dc.identifier.epage | S543, abstract no. P0604 | - |
dc.identifier.isi | WOS:000362830600321 | - |
dc.publisher.place | The Netherlands | - |
dc.identifier.issnl | 0168-8278 | - |