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Conference Paper: Fatty acid binding protein 4 initiates cartilage degeneration through inducing the cytokine expressions of macrophage - an in-vitro study
Title | Fatty acid binding protein 4 initiates cartilage degeneration through inducing the cytokine expressions of macrophage - an in-vitro study |
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Authors | |
Issue Date | 2015 |
Citation | The 35th Annual Congress of The Hong Kong Orthopaedic Association (HKOA 2015), Hong Kong, 6-8 November 2015, p. 49, abstract no. 3.4 How to Cite? |
Abstract | INTRODUCTION: Adipokine-induced inflammation plays roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP-4) is a novel adipokine that has been found to influence both metabolic and inflammatory pathways via the interaction with macrophage. METHODS: Murine macrophage (RAW 264.7) and primary murine chondrocyte (isolated from C57/Bl6 mice) were cultured. In all, 5 concentrations of murine FABP-4 (0, 16, 80, 400, and 2000 ng/mL) were supplemented to the macrophage culture medium and interacted for 24 hours. The conditioned medium was subsequently incubated with chondrocyte for another 24 hours. Gene expressions of cytokines interleukin (IL)-1β, IL-6, and tumour necrosis factor–alpha (TNF-α) of macrophage, and matrix metalloproteinase (MMP)–2, MMP-3, and MMP-13 of chondrocytes were detected using real-time polymerase chain reaction. RESULTS: The mRNA expressions of all targets showed an increasing trend with the rise of FABP-4 concentration, with the expression folds of control (0 ng/mL FABP-4) being 1.62, 1.87, 2.80, 9.15 for IL-1β; 2.62, 4.25, 12.99, 83.71 for IL-6; 1.84, 2.07, 2.35, 2.27 for TNF-α; 0.88, 1.07, 1.40, 2.13 for MMP-2; 1.15, 1.57, 1.75, 2.21 for MMP-3; and 1.03, 1.66, 1.73, 4.63 for MMP-13 under the concentrations of 16, 80, 400 and 2000 ng/mL FABP-4, respectively. CONCLUSIONS: The FABP-4 initiates cartilage degeneration through inducing the cytokine expressions of macrophage. |
Description | Free Paper Session 3 - Basic Science 1: no. 3.4 |
Persistent Identifier | http://hdl.handle.net/10722/235139 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, CF | - |
dc.contributor.author | Yan, CH | - |
dc.contributor.author | Wen, C | - |
dc.contributor.author | Chan, PMB | - |
dc.contributor.author | Li, T | - |
dc.contributor.author | Chiu, PKY | - |
dc.date.accessioned | 2016-10-14T13:51:29Z | - |
dc.date.available | 2016-10-14T13:51:29Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | The 35th Annual Congress of The Hong Kong Orthopaedic Association (HKOA 2015), Hong Kong, 6-8 November 2015, p. 49, abstract no. 3.4 | - |
dc.identifier.uri | http://hdl.handle.net/10722/235139 | - |
dc.description | Free Paper Session 3 - Basic Science 1: no. 3.4 | - |
dc.description.abstract | INTRODUCTION: Adipokine-induced inflammation plays roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP-4) is a novel adipokine that has been found to influence both metabolic and inflammatory pathways via the interaction with macrophage. METHODS: Murine macrophage (RAW 264.7) and primary murine chondrocyte (isolated from C57/Bl6 mice) were cultured. In all, 5 concentrations of murine FABP-4 (0, 16, 80, 400, and 2000 ng/mL) were supplemented to the macrophage culture medium and interacted for 24 hours. The conditioned medium was subsequently incubated with chondrocyte for another 24 hours. Gene expressions of cytokines interleukin (IL)-1β, IL-6, and tumour necrosis factor–alpha (TNF-α) of macrophage, and matrix metalloproteinase (MMP)–2, MMP-3, and MMP-13 of chondrocytes were detected using real-time polymerase chain reaction. RESULTS: The mRNA expressions of all targets showed an increasing trend with the rise of FABP-4 concentration, with the expression folds of control (0 ng/mL FABP-4) being 1.62, 1.87, 2.80, 9.15 for IL-1β; 2.62, 4.25, 12.99, 83.71 for IL-6; 1.84, 2.07, 2.35, 2.27 for TNF-α; 0.88, 1.07, 1.40, 2.13 for MMP-2; 1.15, 1.57, 1.75, 2.21 for MMP-3; and 1.03, 1.66, 1.73, 4.63 for MMP-13 under the concentrations of 16, 80, 400 and 2000 ng/mL FABP-4, respectively. CONCLUSIONS: The FABP-4 initiates cartilage degeneration through inducing the cytokine expressions of macrophage. | - |
dc.language | eng | - |
dc.relation.ispartof | Annual Congress of The Hong Kong Orthopaedic Association, HKOA 2015 | - |
dc.title | Fatty acid binding protein 4 initiates cartilage degeneration through inducing the cytokine expressions of macrophage - an in-vitro study | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Yan, CH: yanchoi@hku.hk | - |
dc.identifier.email | Wen, C: paulwen@hku.hk | - |
dc.identifier.email | Chan, PMB: pmbchan@HKUCC-COM.hku.hk | - |
dc.identifier.email | Chiu, PKY: pkychiu@hkucc.hku.hk | - |
dc.identifier.authority | Yan, CH=rp00303 | - |
dc.identifier.authority | Chiu, PKY=rp00379 | - |
dc.identifier.hkuros | 269192 | - |
dc.identifier.hkuros | 270226 | - |
dc.identifier.spage | 49, abstract no. 3.4 | - |
dc.identifier.epage | 49, abstract no. 3.4 | - |