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Article: Molecular prevalence and subtyping of Cryptosporidium hominis among captive long-tailed macaques (Macaca fascicularis) and rhesus macaques (Macaca mulatta) from Hainan Island, southern China

TitleMolecular prevalence and subtyping of Cryptosporidium hominis among captive long-tailed macaques (Macaca fascicularis) and rhesus macaques (Macaca mulatta) from Hainan Island, southern China
Authors
KeywordsCryptosporidium
Gp60
Macaca fascicularis
Macaca mulatta
SSU rRNA
Issue Date2019
PublisherBioMed Central Ltd.. The Journal's web site is located at http://www.parasitesandvectors.com/home
Citation
Parasites & Vectors, 2019, v. 12, p. article no. 192 How to Cite?
AbstractBackground: Cryptosporidium is an important zoonotic parasite that is commonly found in non-human primates (NHPs). Consequently, there is the potential for transmission of this pathogen from NHPs to humans. However, molecular characterization of the isolates of Cryptosporidium from NHPs remains relatively poor. The aim of the present work was to (i) determine the prevalence; and (ii) perform a genetic characterization of the Cryptosporidium isolated from captive Macaca fascicularis and M. mulatta on Hainan Island in southern China. Methods: A total of 223 fresh fecal samples were collected from captive M. fascicularis (n = 193) and M. mulatta (n = 30). The fecal specimens were examined for the presence of Cryptosporidium spp. by polymerase chain reaction (PCR) and sequencing of the partial small subunit (SSU) rRNA gene. The Cryptosporidium-positive specimens were subtyped by analyzing the 60-kDa glycoprotein (gp60) gene sequence. Results: Cryptosporidium spp. were detected in 5.7% (11/193) of M. fascicularis. All of the 11 Cryptosporidium isolates were identified as C. hominis. Subtyping of nine of these isolates identified four unique gp60 subtypes of C. hominis. These included IaA20R3a (n = 1), IoA17a (n = 1), IoA17b (n = 1), and IiA17 (n = 6). Notably, subtypes IaA20R3a, IoA17a, and IoA17b were novel subtypes which have not been reported previously. Conclusions: To our knowledge, this is the first reported detection of Cryptosporidium in captive M. fascicularis from Hainan Island. The molecular characteristics and subtypes of the isolates here provide novel insights into the genotypic variation in C. hominis. © 2019 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/273968
ISSN
2017 Impact Factor: 3.163
2015 SCImago Journal Rankings: 1.728
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhao, W-
dc.contributor.authorZhou, H-
dc.contributor.authorJin, H-
dc.contributor.authorLiu, M-
dc.contributor.authorQiu, M-
dc.contributor.authorLi, L-
dc.contributor.authorYin, F-
dc.contributor.authorChan, JFW-
dc.contributor.authorLu, G-
dc.date.accessioned2019-08-18T14:52:22Z-
dc.date.available2019-08-18T14:52:22Z-
dc.date.issued2019-
dc.identifier.citationParasites & Vectors, 2019, v. 12, p. article no. 192-
dc.identifier.issn1756-3305-
dc.identifier.urihttp://hdl.handle.net/10722/273968-
dc.description.abstractBackground: Cryptosporidium is an important zoonotic parasite that is commonly found in non-human primates (NHPs). Consequently, there is the potential for transmission of this pathogen from NHPs to humans. However, molecular characterization of the isolates of Cryptosporidium from NHPs remains relatively poor. The aim of the present work was to (i) determine the prevalence; and (ii) perform a genetic characterization of the Cryptosporidium isolated from captive Macaca fascicularis and M. mulatta on Hainan Island in southern China. Methods: A total of 223 fresh fecal samples were collected from captive M. fascicularis (n = 193) and M. mulatta (n = 30). The fecal specimens were examined for the presence of Cryptosporidium spp. by polymerase chain reaction (PCR) and sequencing of the partial small subunit (SSU) rRNA gene. The Cryptosporidium-positive specimens were subtyped by analyzing the 60-kDa glycoprotein (gp60) gene sequence. Results: Cryptosporidium spp. were detected in 5.7% (11/193) of M. fascicularis. All of the 11 Cryptosporidium isolates were identified as C. hominis. Subtyping of nine of these isolates identified four unique gp60 subtypes of C. hominis. These included IaA20R3a (n = 1), IoA17a (n = 1), IoA17b (n = 1), and IiA17 (n = 6). Notably, subtypes IaA20R3a, IoA17a, and IoA17b were novel subtypes which have not been reported previously. Conclusions: To our knowledge, this is the first reported detection of Cryptosporidium in captive M. fascicularis from Hainan Island. The molecular characteristics and subtypes of the isolates here provide novel insights into the genotypic variation in C. hominis. © 2019 The Author(s).-
dc.languageeng-
dc.publisherBioMed Central Ltd.. The Journal's web site is located at http://www.parasitesandvectors.com/home-
dc.relation.ispartofParasites & Vectors-
dc.rightsParasites & Vectors. Copyright © BioMed Central Ltd..-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCryptosporidium-
dc.subjectGp60-
dc.subjectMacaca fascicularis-
dc.subjectMacaca mulatta-
dc.subjectSSU rRNA-
dc.titleMolecular prevalence and subtyping of Cryptosporidium hominis among captive long-tailed macaques (Macaca fascicularis) and rhesus macaques (Macaca mulatta) from Hainan Island, southern China-
dc.typeArticle-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.authorityChan, JFW=rp01736-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/s13071-019-3449-0-
dc.identifier.pmid31039801-
dc.identifier.scopuseid_2-s2.0-85065148916-
dc.identifier.hkuros301249-
dc.identifier.volume12-
dc.identifier.spagearticle no. 192-
dc.identifier.epagearticle no. 192-
dc.identifier.isiWOS:000466387300002-
dc.publisher.placeUnited Kingdom-

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