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Article: Effect of Silver Nitrate and Sodium Fluoride with Tri-Calcium Phosphate on Streptococcus mutans and Demineralised Dentine

TitleEffect of Silver Nitrate and Sodium Fluoride with Tri-Calcium Phosphate on Streptococcus mutans and Demineralised Dentine
Authors
KeywordsDentine caries
Silver nitrate
Sodium fluoride
fTCP
Issue Date2018
PublisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms
Citation
International Journal of Molecular Sciences, 2018, v. 19 n. 5, article no. 1288 How to Cite?
AbstractThis study investigated the effect of 25% silver nitrate (AgNO3) and 5% sodium fluoride (NaF) varnish with functionalized tri-calcium phosphate (fTCP) on a Streptococcus mutans (S. mutans) biofilm and dentine caries lesion. Demineralised dentine specimens were treated with 25% AgNO3 and 5% NaF + fTCP (Group 1), 25% AgNO3 and 5% NaF (Group 2), 25% AgNO3 (Group 3), or water (Group 4). The specimens were subjected to a S. mutans biofilm challenge after treatment. The biofilm was then studied via scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony forming units (CFU). The specimens were assessed by micro-computed tomography, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). SEM and CLSM revealed less biofilm in Groups 1 to 3. The log10 CFU of Groups 1 to 4 were 4.5 ± 0.7, 4.4 ± 0.9, 4.4 ± 0.9, and 6.7 ± 1.0, respectively (Groups 1, 2, 3 < 4, p < 0.001). The lesion depths of Groups 1 to 4 were 212.6 ± 20.1 µm, 280.8 ± 51.6 µm, 402.5 ± 61.7 µm, and 497.4 ± 67.2 µm, respectively (Groups 1 < 2 < 3 < 4, p < 0.001). XRD demonstrated silver chloride formation in Groups 1, 2, and 3. FTIR found the amide I: HPO42− values of the four groups were 0.22 ± 0.05, 0.25 ± 0.05, 0.41 ± 0.12, and 0.64 ± 0.14, respectively (Groups 1, 2 < 3 < 4; p < 0.001). In conclusion, this study revealed that AgNO3 and NaF + fTCP reduced the damage of dentine caries by cariogenic biofilm.
Persistent Identifierhttp://hdl.handle.net/10722/254626
ISSN
2023 Impact Factor: 4.9
2023 SCImago Journal Rankings: 1.179
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYu, OY-
dc.contributor.authorZhao, IS-
dc.contributor.authorMei, ML-
dc.contributor.authorLo, ECM-
dc.contributor.authorChu, CH-
dc.date.accessioned2018-06-21T01:03:35Z-
dc.date.available2018-06-21T01:03:35Z-
dc.date.issued2018-
dc.identifier.citationInternational Journal of Molecular Sciences, 2018, v. 19 n. 5, article no. 1288-
dc.identifier.issn1422-0067-
dc.identifier.urihttp://hdl.handle.net/10722/254626-
dc.description.abstractThis study investigated the effect of 25% silver nitrate (AgNO3) and 5% sodium fluoride (NaF) varnish with functionalized tri-calcium phosphate (fTCP) on a Streptococcus mutans (S. mutans) biofilm and dentine caries lesion. Demineralised dentine specimens were treated with 25% AgNO3 and 5% NaF + fTCP (Group 1), 25% AgNO3 and 5% NaF (Group 2), 25% AgNO3 (Group 3), or water (Group 4). The specimens were subjected to a S. mutans biofilm challenge after treatment. The biofilm was then studied via scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony forming units (CFU). The specimens were assessed by micro-computed tomography, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). SEM and CLSM revealed less biofilm in Groups 1 to 3. The log10 CFU of Groups 1 to 4 were 4.5 ± 0.7, 4.4 ± 0.9, 4.4 ± 0.9, and 6.7 ± 1.0, respectively (Groups 1, 2, 3 < 4, p < 0.001). The lesion depths of Groups 1 to 4 were 212.6 ± 20.1 µm, 280.8 ± 51.6 µm, 402.5 ± 61.7 µm, and 497.4 ± 67.2 µm, respectively (Groups 1 < 2 < 3 < 4, p < 0.001). XRD demonstrated silver chloride formation in Groups 1, 2, and 3. FTIR found the amide I: HPO42− values of the four groups were 0.22 ± 0.05, 0.25 ± 0.05, 0.41 ± 0.12, and 0.64 ± 0.14, respectively (Groups 1, 2 < 3 < 4; p < 0.001). In conclusion, this study revealed that AgNO3 and NaF + fTCP reduced the damage of dentine caries by cariogenic biofilm.-
dc.languageeng-
dc.publisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms-
dc.relation.ispartofInternational Journal of Molecular Sciences-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectDentine caries-
dc.subjectSilver nitrate-
dc.subjectSodium fluoride-
dc.subjectfTCP-
dc.titleEffect of Silver Nitrate and Sodium Fluoride with Tri-Calcium Phosphate on Streptococcus mutans and Demineralised Dentine-
dc.typeArticle-
dc.identifier.emailYu, OY: ollieyu@hku.hk-
dc.identifier.emailMei, ML: mei1123@hku.hk-
dc.identifier.emailLo, ECM: edward-lo@hku.hk-
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.authorityYu, OY=rp02658-
dc.identifier.authorityMei, ML=rp01840-
dc.identifier.authorityLo, ECM=rp00015-
dc.identifier.authorityChu, CH=rp00022-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/ijms19051288-
dc.identifier.pmid29693627-
dc.identifier.pmcidPMC5983598-
dc.identifier.scopuseid_2-s2.0-85046108864-
dc.identifier.hkuros285747-
dc.identifier.volume19-
dc.identifier.issue5-
dc.identifier.spagearticle no. 1288-
dc.identifier.epagearticle no. 1288-
dc.identifier.isiWOS:000435297000024-
dc.publisher.placeSwitzerland-
dc.identifier.issnl1422-0067-

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