File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Caries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions

TitleCaries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions
Authors
KeywordsCaries
Dentine
Silver diamine fluoride
Sodium fluoride
Issue Date2018
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
Citation
Journal of Dentistry, 2018, v. 78, p. 65-71 How to Cite?
AbstractObjectives: To investigate the remineralising effect and bacterial growth inhibition of 38% silver diamine fluoride (SDF) solution and 5% sodium fluoride (NaF) varnish on artificial dentine caries lesions. Methods: Demineralised dentine blocks were treated with SDF + NaF (Group 1), SDF (Group 2), NaF (Group 3) and water (Group 4) and subjected to a Streptococcus mutans biofilm challenge. Lesion depth, precipitates’ characteristics and matrix (collagen)-to-mineral ratio were evaluated by micro-computer tomography (micro-CT), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. The biofilm kinetics, viability and topography were assessed by counts of colony forming units (CFUs), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), respectively. Data were analysed by two-way ANOVA test. Results: The lesion depths of Groups 1–4 were 170 ± 28 μm, 160 ± 32 μm, 353 ± 38 μm and 449 ± 24 μm, respectively. The addition of NaF to SDF did not show better remineralisation than SDF (p = 0.491). Metallic silver and silver chloride were found in Groups 1 and 2. The amide I-to-hydrogen phosphate ratios of the four groups were 0.14 ± 0.02, 0.14 ± 0.01, 0.29 ± 0.05 and 0.49 ± 0.16, respectively, and the addition of NaF to SDF did not offer better protection against collagen exposure than SDF (p = 0.986). The Log10 CFUs of Groups 1–4 were 5.75 ± 0.56, 4.49 ± 0.57, 6.55 ± 0.39 and 6.40 ± 0.38, respectively. The presence of NaF reduced the antibacterial effect of SDF (p < 0.001). The SEM and CLSM images supported the findings. Conclusion: Application of SDF with or without NaF reduced the demineralisation of dentine caries, but SDF exerted stronger inhibition of biofilm growth than SDF with NaF. Clinical significance: NaF varnish affects the antibacterialeffects of SDF, the adjunctive application of SDF solution and NaF varnish is not recommended to arrest dentine caries in clinic. © 2018 Elsevier Ltd
Persistent Identifierhttp://hdl.handle.net/10722/263961
ISSN
2023 Impact Factor: 4.8
2023 SCImago Journal Rankings: 1.313
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYu, OY-
dc.contributor.authorZhao, IS-
dc.contributor.authorMei, L-
dc.contributor.authorLo, ECM-
dc.contributor.authorChu, CH-
dc.date.accessioned2018-10-22T07:47:16Z-
dc.date.available2018-10-22T07:47:16Z-
dc.date.issued2018-
dc.identifier.citationJournal of Dentistry, 2018, v. 78, p. 65-71-
dc.identifier.issn0300-5712-
dc.identifier.urihttp://hdl.handle.net/10722/263961-
dc.description.abstractObjectives: To investigate the remineralising effect and bacterial growth inhibition of 38% silver diamine fluoride (SDF) solution and 5% sodium fluoride (NaF) varnish on artificial dentine caries lesions. Methods: Demineralised dentine blocks were treated with SDF + NaF (Group 1), SDF (Group 2), NaF (Group 3) and water (Group 4) and subjected to a Streptococcus mutans biofilm challenge. Lesion depth, precipitates’ characteristics and matrix (collagen)-to-mineral ratio were evaluated by micro-computer tomography (micro-CT), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. The biofilm kinetics, viability and topography were assessed by counts of colony forming units (CFUs), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), respectively. Data were analysed by two-way ANOVA test. Results: The lesion depths of Groups 1–4 were 170 ± 28 μm, 160 ± 32 μm, 353 ± 38 μm and 449 ± 24 μm, respectively. The addition of NaF to SDF did not show better remineralisation than SDF (p = 0.491). Metallic silver and silver chloride were found in Groups 1 and 2. The amide I-to-hydrogen phosphate ratios of the four groups were 0.14 ± 0.02, 0.14 ± 0.01, 0.29 ± 0.05 and 0.49 ± 0.16, respectively, and the addition of NaF to SDF did not offer better protection against collagen exposure than SDF (p = 0.986). The Log10 CFUs of Groups 1–4 were 5.75 ± 0.56, 4.49 ± 0.57, 6.55 ± 0.39 and 6.40 ± 0.38, respectively. The presence of NaF reduced the antibacterial effect of SDF (p < 0.001). The SEM and CLSM images supported the findings. Conclusion: Application of SDF with or without NaF reduced the demineralisation of dentine caries, but SDF exerted stronger inhibition of biofilm growth than SDF with NaF. Clinical significance: NaF varnish affects the antibacterialeffects of SDF, the adjunctive application of SDF solution and NaF varnish is not recommended to arrest dentine caries in clinic. © 2018 Elsevier Ltd-
dc.languageeng-
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent-
dc.relation.ispartofJournal of Dentistry-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCaries-
dc.subjectDentine-
dc.subjectSilver diamine fluoride-
dc.subjectSodium fluoride-
dc.titleCaries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions-
dc.typeArticle-
dc.identifier.emailMei, L: mei1123@hku.hk-
dc.identifier.emailLo, ECM: edward-lo@hku.hk-
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.authorityMei, L=rp01840-
dc.identifier.authorityLo, ECM=rp00015-
dc.identifier.authorityChu, CH=rp00022-
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.jdent.2018.08.007-
dc.identifier.pmid30114443-
dc.identifier.scopuseid_2-s2.0-85051649784-
dc.identifier.hkuros295768-
dc.identifier.volume78-
dc.identifier.spage65-
dc.identifier.epage71-
dc.identifier.isiWOS:000446678100008-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0300-5712-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats