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Article: High-precision tumor resection down to few-cell level guided by NIR-IIb molecular fluorescence imaging

TitleHigh-precision tumor resection down to few-cell level guided by NIR-IIb molecular fluorescence imaging
Authors
Keywordsfluorescence imaging
image-guided surgery
molecular imaging
NIR-IIb imaging
short-wave infrared
Issue Date2022
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2022, v. 119, n. 15, article no. e2123111119 How to Cite?
AbstractIn vivo fluorescence/luminescence imaging in the near-infrared-IIb (NIR-IIb, 1,500 to 1,700 nm) window under <1,000 nm excitation can afford subcentimeter imaging depth without any tissue autofluorescence, promising high-precision intraoperative navigation in the clinic. Here, we developed a compact imager for concurrent visible photographic and NIR-II (1,000 to 3,000 nm) fluorescence imaging for preclinical image-guided surgery. Biocompatible erbium-based rare-earth nanoparticles (ErNPs) with bright down-conversion luminescence in the NIR-IIb window were conjugated to TRC105 antibody for molecular imaging of CD105 angiogenesis markers in 4T1 murine breast tumors. Under a ∼940 ± 38 nm light-emitting diode (LED) excitation, NIR-IIb imaging of 1,500- to 1,700-nm emission afforded noninvasive tumor-to-normal tissue (T/NT) signal ratios of ∼40 before surgery and an ultrahigh intraoperative tumor-to-muscle (T/M) ratio of ∼300, resolving tumor margin unambiguously without interfering background signal from surrounding healthy tissues. High-resolution imaging resolved small numbers of residual cancer cells during surgery, allowing thorough and nonexcessive tumor removal at the few-cell level. NIR-IIb molecular imaging afforded 10-times-higher and 100-times-higher T/NT and T/M ratios, respectively, than imaging with IRDye800CW-TRC105 in the ∼900- to 1,300-nm range. The vastly improved resolution of tumor margin and diminished background open a paradigm of molecular imaging-guided surgery.
Persistent Identifierhttp://hdl.handle.net/10722/325693
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Feifei-
dc.contributor.authorQu, Liangqiong-
dc.contributor.authorRen, Fuqiang-
dc.contributor.authorBaghdasaryan, Ani-
dc.contributor.authorJiang, Yingying-
dc.contributor.authorHsu, Ru Siou-
dc.contributor.authorLiang, Peng-
dc.contributor.authorLi, Jiachen-
dc.contributor.authorZhu, Guanzhou-
dc.contributor.authorMa, Zhuoran-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-02-27T07:35:29Z-
dc.date.available2023-02-27T07:35:29Z-
dc.date.issued2022-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2022, v. 119, n. 15, article no. e2123111119-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/325693-
dc.description.abstractIn vivo fluorescence/luminescence imaging in the near-infrared-IIb (NIR-IIb, 1,500 to 1,700 nm) window under <1,000 nm excitation can afford subcentimeter imaging depth without any tissue autofluorescence, promising high-precision intraoperative navigation in the clinic. Here, we developed a compact imager for concurrent visible photographic and NIR-II (1,000 to 3,000 nm) fluorescence imaging for preclinical image-guided surgery. Biocompatible erbium-based rare-earth nanoparticles (ErNPs) with bright down-conversion luminescence in the NIR-IIb window were conjugated to TRC105 antibody for molecular imaging of CD105 angiogenesis markers in 4T1 murine breast tumors. Under a ∼940 ± 38 nm light-emitting diode (LED) excitation, NIR-IIb imaging of 1,500- to 1,700-nm emission afforded noninvasive tumor-to-normal tissue (T/NT) signal ratios of ∼40 before surgery and an ultrahigh intraoperative tumor-to-muscle (T/M) ratio of ∼300, resolving tumor margin unambiguously without interfering background signal from surrounding healthy tissues. High-resolution imaging resolved small numbers of residual cancer cells during surgery, allowing thorough and nonexcessive tumor removal at the few-cell level. NIR-IIb molecular imaging afforded 10-times-higher and 100-times-higher T/NT and T/M ratios, respectively, than imaging with IRDye800CW-TRC105 in the ∼900- to 1,300-nm range. The vastly improved resolution of tumor margin and diminished background open a paradigm of molecular imaging-guided surgery.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectfluorescence imaging-
dc.subjectimage-guided surgery-
dc.subjectmolecular imaging-
dc.subjectNIR-IIb imaging-
dc.subjectshort-wave infrared-
dc.titleHigh-precision tumor resection down to few-cell level guided by NIR-IIb molecular fluorescence imaging-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1073/pnas.2123111119-
dc.identifier.pmid35380898-
dc.identifier.pmcidPMC9169804-
dc.identifier.scopuseid_2-s2.0-85127695046-
dc.identifier.volume119-
dc.identifier.issue15-
dc.identifier.spagearticle no. e2123111119-
dc.identifier.epagearticle no. e2123111119-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000800045000008-

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