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Article: The Anti-Caries Effects of a Novel Peptide on Dentine Caries: An In Vitro Study
Title | The Anti-Caries Effects of a Novel Peptide on Dentine Caries: An In Vitro Study |
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Authors | |
Keywords | antimicrobial caries demineralization peptides prevention remineralization |
Issue Date | 14-Sep-2023 |
Publisher | MDPI |
Citation | International Journal of Molecular Sciences, 2023, v. 24, n. 18 How to Cite? |
Abstract | This study aimed to investigate the antibiofilm and remineralising effects of peptide GAPI on artificial dentin caries. After creating artificial carious lesions, eighty dentine blocks were randomly assigned for treatment twice daily with GAPI (GAPI group) or deionised water (control group). Both groups underwent a 7-day biochemical cycle. Scanning electron microscopy (SEM) showed S. mutans with damaged structures that partially covered the dentine in the GAPI group. The dead-live ratios for the GAPI and control groups were 0.77 +/- 0.13 and 0.37 +/- 0.09 (p < 0.001). The log colony-forming units for the GAPI and control groups were 7.45 +/- 0.32 and 8.74 +/- 0.50 (p < 0.001), respectively. The lesion depths for the GAPI and control groups were 151 +/- 18 mu m and 214 +/- 15 mu m (p < 0.001), respectively. The mineral losses for the GAPI and control groups were 0.91 +/- 0.07 gHAcm(-3) and 1.01 +/- 0.07 gHAcm(-3) (p = 0.01), respectively. The hydrogen-to-amide I ratios for the GAPI and control groups were 2.92 +/- 0.82 and 1.83 +/- 0.73 (p = 0.014), respectively. SEM micrographs revealed fewer exposed dentine collagen fibres in the GAPI group compared to those in the control group. Furthermore, X-ray diffraction (XRD) patterns indicated that the hydroxyapatite in the GAPI group was more crystallised than that in the control group. This study demonstrated GAPI's antibiofilm and remineralising effects on artificial dentin caries. |
Persistent Identifier | http://hdl.handle.net/10722/340916 |
ISSN | 2023 Impact Factor: 4.9 2023 SCImago Journal Rankings: 1.179 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhang, Lili | - |
dc.contributor.author | Niu, Yun | - |
dc.contributor.author | Yu, Yiru Ollie | - |
dc.contributor.author | Yin, Xiaoxue Iris | - |
dc.contributor.author | Mei, L May | - |
dc.contributor.author | Chu, Hung Chun | - |
dc.date.accessioned | 2024-03-11T10:48:16Z | - |
dc.date.available | 2024-03-11T10:48:16Z | - |
dc.date.issued | 2023-09-14 | - |
dc.identifier.citation | International Journal of Molecular Sciences, 2023, v. 24, n. 18 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/10722/340916 | - |
dc.description.abstract | <p>This study aimed to investigate the antibiofilm and remineralising effects of peptide GAPI on artificial dentin caries. After creating artificial carious lesions, eighty dentine blocks were randomly assigned for treatment twice daily with GAPI (GAPI group) or deionised water (control group). Both groups underwent a 7-day biochemical cycle. Scanning electron microscopy (SEM) showed S. mutans with damaged structures that partially covered the dentine in the GAPI group. The dead-live ratios for the GAPI and control groups were 0.77 +/- 0.13 and 0.37 +/- 0.09 (p < 0.001). The log colony-forming units for the GAPI and control groups were 7.45 +/- 0.32 and 8.74 +/- 0.50 (p < 0.001), respectively. The lesion depths for the GAPI and control groups were 151 +/- 18 mu m and 214 +/- 15 mu m (p < 0.001), respectively. The mineral losses for the GAPI and control groups were 0.91 +/- 0.07 gHAcm(-3) and 1.01 +/- 0.07 gHAcm(-3) (p = 0.01), respectively. The hydrogen-to-amide I ratios for the GAPI and control groups were 2.92 +/- 0.82 and 1.83 +/- 0.73 (p = 0.014), respectively. SEM micrographs revealed fewer exposed dentine collagen fibres in the GAPI group compared to those in the control group. Furthermore, X-ray diffraction (XRD) patterns indicated that the hydroxyapatite in the GAPI group was more crystallised than that in the control group. This study demonstrated GAPI's antibiofilm and remineralising effects on artificial dentin caries.<br></p> | - |
dc.language | eng | - |
dc.publisher | MDPI | - |
dc.relation.ispartof | International Journal of Molecular Sciences | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | antimicrobial | - |
dc.subject | caries | - |
dc.subject | demineralization | - |
dc.subject | peptides | - |
dc.subject | prevention | - |
dc.subject | remineralization | - |
dc.title | The Anti-Caries Effects of a Novel Peptide on Dentine Caries: An In Vitro Study | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/ijms241814076 | - |
dc.identifier.scopus | eid_2-s2.0-85172874101 | - |
dc.identifier.volume | 24 | - |
dc.identifier.issue | 18 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.identifier.isi | WOS:001076788900001 | - |
dc.identifier.issnl | 1422-0067 | - |