Antibacterial and mineralising properties of copper tetraamine fluoride on dentine caries: A laboratory study

Abstract

<p>Objective: To investigate the antibacterial, mineralising, and discolouring properties of copper tetraamine fluoride (CTF) on artificial dentine caries. Method: Demineralised human dentine blocks were treated with CTF, silver diamine fluoride (SDF, positive control) and water (negative control) before they were subjected to cariogenic challenge with Streptococcus mutans biofilm. The morphology, viability, and growth kinetics of the biofilm were assessed by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony-forming unit (CFU) counting. The lesion depths, mineral loss, chemical structure, and crystal characteristics were measured using micro-computed tomography, Fourier transform infrared (FTIR), and X-ray diffraction (XRD). The discolouring property on demineralised dentine was assessed by spectrophotometry. One-way analysis of variance with the Bonferroni post hoc test was performed to assess and compare the data. Results: SEM revealed confluent bacterial growth covering the surface of dentine treated with Water but not CTF and SDF. CLSM showed the dead-to-live ratio of the biofilm treated with CTF, SDF and Water were 0.7 ± 0.1, 0.8 ± 0.1 and 0.5 ± 0.1 (p < 0.001, CTF, SDF>Water). The Log₁₀ CFU values of the biofilm treated with CTF, SDF and Water were 7.8 ± 0.1, 7.8 ± 0.1 and 8.1 ± 0.2 (p < 0.001, CTF, SDF−3) of dentine treated with CTF, SDF and Water were 0.19 ± 0.04, 0.18 ± 0.04 and 0.30 ± 0.04 (p < 0.001, CTF, SDF</p>