File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)

Article: Monochromosome transfer and microarray analysis identify a critical tumor-suppressive region mapping to chromosome 13q14 and THSD1 in esophageal carcinoma

TitleMonochromosome transfer and microarray analysis identify a critical tumor-suppressive region mapping to chromosome 13q14 and THSD1 in esophageal carcinoma
Authors
Issue Date2008
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://mcr.aacrjournals.org/
Citation
Molecular Cancer Research, 2008, v. 6 n. 4, p. 592-603 How to Cite?
AbstractLoss of chromosome 13q regions in esophageal squamous cell carcinoma (ESCC) is a frequent event. Monochromosome transfer approaches provide direct functional evidence for tumor suppression by chromosome 13 in SLMT-1, an ESCC cell line, and identify critical regions at 13q12.3, 13q14.11, and 13q14.3. Differential gene expression profiles of three tumor-suppressing microcell hybrids (MCH) and their tumorigenic parental SLMT-1 cell line were revealed by competitive hybridization using 19k cDNA oligonucleotide microarrays. Nine candidate 13q14 tumor-suppressor genes (TSG), including RB1, showed down-regulation in SLMT-1, compared with NE1, an immortalized normal esophageal epithelial cell line; their average gene expression was restored in MCHs compared with SLMT-1. Reverse transcription-PCR validated gene expression levels in MCHs and a panel of ESCC cell lines. Results suggest that the tumor-suppressing effect is not attributed to RB1, but instead likely involves thrombospondin type I domain-containing 1 (THSD1), a novel candidate TSG mapping to 13q14. Quantitative reverse transcription-PCR detected down-regulation of THSD1 expression in 100% of ESCC and other cancer cell lines. Mechanisms for THSD1 silencing in ESCC involved loss of heterozygosity and promoter hypermethylation, as analyzed by methylation-specific PCR and clonal bisulfite sequencing. Transfection of wild-type THSD1 into SLMT-1 resulted in significant reduction of colony-forming ability, hence providing functional evidence for its growth-suppressive activity. These findings suggest that THSD1 is a good candidate TSG. Copyright © 2008 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/67774
ISSN
2023 Impact Factor: 4.1
2023 SCImago Journal Rankings: 1.660
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKo, JMYen_HK
dc.contributor.authorChan, PLen_HK
dc.contributor.authorYau, WLen_HK
dc.contributor.authorChan, HKen_HK
dc.contributor.authorChan, KCen_HK
dc.contributor.authorYu, ZYen_HK
dc.contributor.authorKwong, FMen_HK
dc.contributor.authorMiller, LDen_HK
dc.contributor.authorLiu, ETen_HK
dc.contributor.authorYang, LCen_HK
dc.contributor.authorLo, PHYen_HK
dc.contributor.authorStanbridge, EJen_HK
dc.contributor.authorTang, JCOen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorLung, MLen_HK
dc.date.accessioned2010-09-06T05:58:08Z-
dc.date.available2010-09-06T05:58:08Z-
dc.date.issued2008en_HK
dc.identifier.citationMolecular Cancer Research, 2008, v. 6 n. 4, p. 592-603en_HK
dc.identifier.issn1541-7786en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67774-
dc.description.abstractLoss of chromosome 13q regions in esophageal squamous cell carcinoma (ESCC) is a frequent event. Monochromosome transfer approaches provide direct functional evidence for tumor suppression by chromosome 13 in SLMT-1, an ESCC cell line, and identify critical regions at 13q12.3, 13q14.11, and 13q14.3. Differential gene expression profiles of three tumor-suppressing microcell hybrids (MCH) and their tumorigenic parental SLMT-1 cell line were revealed by competitive hybridization using 19k cDNA oligonucleotide microarrays. Nine candidate 13q14 tumor-suppressor genes (TSG), including RB1, showed down-regulation in SLMT-1, compared with NE1, an immortalized normal esophageal epithelial cell line; their average gene expression was restored in MCHs compared with SLMT-1. Reverse transcription-PCR validated gene expression levels in MCHs and a panel of ESCC cell lines. Results suggest that the tumor-suppressing effect is not attributed to RB1, but instead likely involves thrombospondin type I domain-containing 1 (THSD1), a novel candidate TSG mapping to 13q14. Quantitative reverse transcription-PCR detected down-regulation of THSD1 expression in 100% of ESCC and other cancer cell lines. Mechanisms for THSD1 silencing in ESCC involved loss of heterozygosity and promoter hypermethylation, as analyzed by methylation-specific PCR and clonal bisulfite sequencing. Transfection of wild-type THSD1 into SLMT-1 resulted in significant reduction of colony-forming ability, hence providing functional evidence for its growth-suppressive activity. These findings suggest that THSD1 is a good candidate TSG. Copyright © 2008 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://mcr.aacrjournals.org/en_HK
dc.relation.ispartofMolecular Cancer Researchen_HK
dc.subject.meshAllelesen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromosome Mappingen_HK
dc.subject.meshChromosome Segregationen_HK
dc.subject.meshChromosomes, Human, Pair 13 - geneticsen_HK
dc.subject.meshDNA Methylation - drug effectsen_HK
dc.subject.meshDeoxycytidine - pharmacologyen_HK
dc.subject.meshEpithelial Cells - drug effects - pathologyen_HK
dc.subject.meshEsophageal Neoplasms - geneticsen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplastic - drug effectsen_HK
dc.subject.meshGenes, Tumor Suppressoren_HK
dc.subject.meshGenome, Human - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydroxamic Acids - pharmacologyen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshMicroarray Analysisen_HK
dc.subject.meshMicrosatellite Repeats - geneticsen_HK
dc.subject.meshPromoter Regions, Genetic - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshThrombospondins - genetics - metabolismen_HK
dc.subject.meshTransfectionen_HK
dc.subject.meshTumor Stem Cell Assayen_HK
dc.titleMonochromosome transfer and microarray analysis identify a critical tumor-suppressive region mapping to chromosome 13q14 and THSD1 in esophageal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailSrivastava, G: gopesh@pathology.hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.emailLung, ML: mlilung@hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authorityLung, ML=rp00300en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1158/1541-7786.MCR-07-0154en_HK
dc.identifier.pmid18403638-
dc.identifier.scopuseid_2-s2.0-42049120045en_HK
dc.identifier.hkuros141625en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-42049120045&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue4en_HK
dc.identifier.spage592en_HK
dc.identifier.epage603en_HK
dc.identifier.isiWOS:000254905600009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKo, JMY=35725559400en_HK
dc.identifier.scopusauthoridPui, LC=23095469900en_HK
dc.identifier.scopusauthoridWing, LY=15119281300en_HK
dc.identifier.scopusauthoridHo, KC=24068351900en_HK
dc.identifier.scopusauthoridKing, CC=23570603000en_HK
dc.identifier.scopusauthoridZhuo, YY=24069465900en_HK
dc.identifier.scopusauthoridFung, MK=23570461000en_HK
dc.identifier.scopusauthoridMiller, LD=7404985394en_HK
dc.identifier.scopusauthoridLiu, ET=7202240109en_HK
dc.identifier.scopusauthoridLi, CY=36065611200en_HK
dc.identifier.scopusauthoridLo, PHY=36762664000en_HK
dc.identifier.scopusauthoridStanbridge, EJ=7103249410en_HK
dc.identifier.scopusauthoridTang, JCO=14056850300en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridSai, WT=7102813116en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridLung, ML=7006411788en_HK
dc.identifier.issnl1541-7786-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats